Genome mining to elucidate antimicrobial and plant growth promoting potentials in Lactic acid bacteria

Genome mining to elucidate antimicrobial and plant growth promoting potentials in Lactic acid bacteria | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Genome mining to elucidate antimicrobial and plant growth promoting potentials in Lactic acid bacteria Christian David Vargas, Lina Marcela Botero, Edwin Rodriguez, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4302676/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 13 Feb, 2025 Read the published version in European Journal of Plant Pathology → Version 1 posted 6 You are reading this latest preprint version Abstract The pangenome of Pediococcus sp. was constructed with five bacterial strains isolated from oat silage produced by smallholder farmers from a region in the highlands of the Colombian Andes. The goal of this work was to use genome mining to elucidate some potential mechanisms of action associated with plant growth promotion and biocontrol in lactic acid bacteria (LAB). Multiple genes were uncovered, including those associated with antibiosis and antimicrobial compound production, genes that induce plant defense and phytohormone activation. Additionally, genome sequencing suggested mechanisms for LAB to protect against abiotic and biotic stress. Furthermore, genes that may improve phosphorus availability and those that might stimulate root growth were present. We then tested some of these findings in vitro. For instance, the biocontrol activity of these strains was evaluated against the fungal pathogens Fusarium oxsyporum f. sp. cubense (Foc R1 and Foc TR4) and the bacterial pathogen Ralstonia solanacearum (Rs). All tested strains showed the ability to significantly inhibit pathogen growth in vitro. The capability of LAB to grow at high concentrations of fusaric acid was demonstrated, suggesting that LAB might have detoxification mechanisms to compete with other microorganisms. Also, the ability of LAB to solubilize phosphate and produce IAA was demonstrated. These two properties might be involved in the plant-growth promotion. Here we show that genomic sequencing can be an efficient tool to identify putative biocontrol or plant-growth promoting related sequences for genome mining. Pangenome Lactic acid bacteria Biocontrol Plant growth promoting bacteria Genome mining Full Text Additional Declarations Supplementary figure 2 is not available with this version. Supplementary Files Supplementarytable1.xlsx Supplementarytable2.xlsx Supplementarytable3.xlsx Cite Share Download PDF Status: Published Journal Publication published 13 Feb, 2025 Read the published version in European Journal of Plant Pathology → Version 1 posted Editorial decision: Minor revisions 01 Sep, 2024 Reviewers agreed at journal 27 Apr, 2024 Reviewers invited by journal 26 Apr, 2024 Editor invited by journal 26 Apr, 2024 Editor assigned by journal 24 Apr, 2024 First submitted to journal 21 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4302676","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":295818204,"identity":"c76924c1-674b-4f60-a5b1-0311b2bc93c1","order_by":0,"name":"Christian David Vargas","email":"","orcid":"","institution":"Corporacion Colombiana de Investigación Agropecuaria: AGROSAVIA","correspondingAuthor":false,"prefix":"","firstName":"Christian","middleName":"David","lastName":"Vargas","suffix":""},{"id":295818205,"identity":"4ab4aae5-03bd-453a-b653-e8aeafe64705","order_by":1,"name":"Lina Marcela 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