The Regulatory Effect of Lycium Barbarum Polysaccharide on I κ B Phosphorylation and NF - κ B Nuclear Translocation in RAW264.7 Cells

Abstract Scope: RAW264.7 cells was used to study the regulatory effect of LBP on I κ B phosphorylation and NF - κ B nuclear translocation. Methods: (1)After 5, 10, 20 and 30 minutes of LBP intervention, Western blotting was used to detect the expression of I κ B and P-I κ B in each group, (2)After 5 minutes of intervention with different doses of LBP,Western blotting was used to detect the expression of P-I κ B and I κ B in the cells of each group, (3)After 30 minutes of LBP intervention, the distribution of NF - κ B nuclear translocation was observed by immunofluorescence, and the number of NF - κ B nuclear translocation was detected by Western blotting.Results : The results of the time-dependent regulation of I κ B phosphorylation by LBP showed that when stimulated by LPS, I κ B phosphorylated rapidly, the level of P-I κ B increased and I κ B decreased, and the level of I κ B increased at each time point in the combined treatment group of LPS (1 μ g / ml) + LBP (25 μ g / ml).After LPS stimulation, the expression of I κ B decreased and the expression of phosphorylated I κ B increased (P < 0.05); after LPS (L μ g / ml) + LBP (25,50 μ g / ml), the level of I κ B increased (P < 0.05).After LPS stimulation, the green fluorescence was mainly distributed in the nuclear area, and after LBP stimulation, the distribution of green fluorescence was slightly increased; in LPS + LBP group, the distribution of green fluorescence in the cytoplasm was increased, and the distribution of green fluorescence in the nucleus was decreased. After LPS stimulation, NF - κ B level in cytoplasm decreased (P < 0.05), and NF - κ B level in nucleus increased significantly (P < 0.05). After LPS (L μ g / ml) + LBP (25, 50, 100 μ g / ml), NF - κ B level in cytoplasm increased (P < 0.05), and NF - κ B level in nucleus decreased (P < 0.05). Conclusion: Collectively, these results suggested that LBP's anti-inflammatory effects were relevant to the interruption of NF-kappa B activation.