Effect of culture medium and cell suspension sieving on somatic embryo formation in plantain cv. 'FHIA 04' (Musa spp. AAAB)

Effect of culture medium and cell suspension sieving on somatic embryo formation in plantain cv. 'FHIA 04' (Musa spp. AAAB) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effect of culture medium and cell suspension sieving on somatic embryo formation in plantain cv. 'FHIA 04' (Musa spp. AAAB) Delia Alvarez, Delia Luisa Alvarez-Vázquez, Maritza Reyes Vega, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6545647/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Somatic embryogenesis is considered an efficient technique for plant propagation, as well as a tool for genetic improvement of different crops. This study aims to determine the effect of the culture medium and size of cell clumps on the formation of somatic embryos in the plantain cultivar 'FHIA 04' ( Musa AAAB). Embryogenic cell suspensions were considered in their multiplication phase and divided in two size ranges (i.e. ≤500 and ˃500 µm) of cellular aggregates. After sieving, the cellular aggregates were transferred onto a plastic mesh that was placed on two culture media for somatic embryo formation: one containing 0.4 mg l − 1 6-benzylaminopurine (6BAP) and 0.3 mg l − 1 indol-3-acetic acid (IAA) as growth regulators and the second one without any plant growth regulator. After 30 days of cultivation in the dark at 25 ± 2ºC, the number of somatic embryos formed on each culture medium was evaluated, as well as the effects on their maturation and germination. The combination of embryogenic cellular aggregate with size ≤ 500 µm and the culture medium without growth regulators resulted in more somatic embryo formation, better maturation, and higher germination of the plantain cultivar 'FHIA-04'. cellular aggregates cell culture somatic embryogenesis Musa plantain Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6545647","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":453117552,"identity":"d7f880b0-be82-4ba9-9c49-c7f364431369","order_by":0,"name":"Delia Alvarez","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0002-8186-9001","institution":"Catholic University of Louvain: Universite Catholique de Louvain","correspondingAuthor":true,"prefix":"","firstName":"Delia","middleName":"","lastName":"Alvarez","suffix":""},{"id":453117553,"identity":"c134feeb-018f-4d02-a7a4-18b23689fbd0","order_by":1,"name":"Delia Luisa Alvarez-Vázquez","email":"","orcid":"","institution":"Catholic University of Louvain: Universite Catholique de Louvain","correspondingAuthor":false,"prefix":"","firstName":"Delia","middleName":"Luisa","lastName":"Alvarez-Vázquez","suffix":""},{"id":453117554,"identity":"0eb134cb-6ee6-4b58-b579-ca754c52e495","order_by":2,"name":"Maritza Reyes Vega","email":"","orcid":"","institution":"Institute of Plant Biology and Biotechnology","correspondingAuthor":false,"prefix":"","firstName":"Maritza","middleName":"Reyes","lastName":"Vega","suffix":""},{"id":453117555,"identity":"e166bcc9-cf74-499e-a58b-f5145782ddc7","order_by":3,"name":"Mónica Garcés Ruiz","email":"","orcid":"","institution":"Catholic University of Louvain: Universite Catholique de Louvain","correspondingAuthor":false,"prefix":"","firstName":"Mónica","middleName":"Garcés","lastName":"Ruiz","suffix":""},{"id":453117556,"identity":"94595686-bf57-4180-a3da-51fd2d9e2c1a","order_by":4,"name":"Rafael Gómez Kosky","email":"","orcid":"","institution":"INIA: Instituto de Investigaciones Agropecuarias","correspondingAuthor":false,"prefix":"","firstName":"Rafael","middleName":"Gómez","lastName":"Kosky","suffix":""},{"id":453117557,"identity":"2ffb71ca-57a7-4955-93c3-0bdf0c25d3f2","order_by":5,"name":"Stephane Declerck","email":"","orcid":"","institution":"Catholic University of Louvain: Universite Catholique de Louvain","correspondingAuthor":false,"prefix":"","firstName":"Stephane","middleName":"","lastName":"Declerck","suffix":""},{"id":453117558,"identity":"88fc2330-c466-4281-9ba3-9053df1ae55b","order_by":6,"name":"Bart Panis","email":"","orcid":"","institution":"Alliance of Bioversity International and CIAT: Alliance of Bioversity International and International Center for Tropical Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Bart","middleName":"","lastName":"Panis","suffix":""},{"id":453117559,"identity":"89fb2516-b36e-4127-a47c-59a56480700e","order_by":7,"name":"Leyanes García Aguila","email":"","orcid":"","institution":"Institute of Plant Biology and Biotechnology","correspondingAuthor":false,"prefix":"","firstName":"Leyanes","middleName":"García","lastName":"Aguila","suffix":""},{"id":453117560,"identity":"fa72c802-f463-4fea-a3e3-e1bcbc11b4f3","order_by":8,"name":"Idalmis Bermúdez Caraballoso","email":"","orcid":"","institution":"IPBB: Institute of Plant Biology and Biotechnology","correspondingAuthor":false,"prefix":"","firstName":"Idalmis","middleName":"Bermúdez","lastName":"Caraballoso","suffix":""}],"badges":[],"createdAt":"2025-04-28 08:35:52","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6545647/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6545647/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":84300408,"identity":"670cc2be-f66d-454d-bdec-9bfaaf4ae4c6","added_by":"auto","created_at":"2025-06-10 10:27:46","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":649468,"visible":true,"origin":"","legend":"","description":"","filename":"EffectofMediumandCellSuspensionSievingonSE.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6545647/v1_covered_3c603f83-871f-47bf-ac1c-52ed64f60ba8.pdf"}],"financialInterests":"","formattedTitle":"Effect of culture medium and cell suspension sieving on somatic embryo formation in plantain cv. 'FHIA 04' (Musa spp. AAAB)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"cellular aggregates, cell culture, somatic embryogenesis, Musa, plantain","lastPublishedDoi":"10.21203/rs.3.rs-6545647/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6545647/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSomatic embryogenesis is considered an efficient technique for plant propagation, as well as a tool for genetic improvement of different crops. This study aims to determine the effect of the culture medium and size of cell clumps on the formation of somatic embryos in the plantain cultivar 'FHIA 04' (\u003cem\u003eMusa\u003c/em\u003e AAAB). Embryogenic cell suspensions were considered in their multiplication phase and divided in two size ranges (i.e. \u0026le;500 and ˃500 \u0026micro;m) of cellular aggregates. After sieving, the cellular aggregates were transferred onto a plastic mesh that was placed on two culture media for somatic embryo formation: one containing 0.4 mg l\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e 6-benzylaminopurine (6BAP) and 0.3 mg l\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e indol-3-acetic acid (IAA) as growth regulators and the second one without any plant growth regulator. After 30 days of cultivation in the dark at 25\u0026thinsp;\u0026plusmn;\u0026thinsp;2\u0026ordm;C, the number of somatic embryos formed on each culture medium was evaluated, as well as the effects on their maturation and germination. The combination of embryogenic cellular aggregate with size\u0026thinsp;\u0026le;\u0026thinsp;500 \u0026micro;m and the culture medium without growth regulators resulted in more somatic embryo formation, better maturation, and higher germination of the plantain cultivar 'FHIA-04'.\u003c/p\u003e","manuscriptTitle":"Effect of culture medium and cell suspension sieving on somatic embryo formation in plantain cv. 'FHIA 04' (Musa spp. AAAB)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-05-12 08:37:47","doi":"10.21203/rs.3.rs-6545647/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"394e9d15-6800-4583-86be-5e35385bcb07","owner":[],"postedDate":"May 12th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-06-10T10:19:38+00:00","versionOfRecord":[],"versionCreatedAt":"2025-05-12 08:37:47","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6545647","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6545647","identity":"rs-6545647","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}