Aurora-A Regulating Cervical Cancer Invasion and Metastasis through ARPC4

Aurora-A Regulating Cervical Cancer Invasion and Metastasis through ARPC4 | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Aurora-A Regulating Cervical Cancer Invasion and Metastasis through ARPC4 Yaqing Yue, Zhaoxia Mu, Xibo Wang, Yan Liu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3884961/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective： To investigate the impact of ARPC4 knockdown on cervical cancer cells with Aurora-A overexpression in terms of proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). Methods: Gene expression profiling by RNA-seq, qPCR, and Western blotting were used to identify genes potentially regulated by Aurora-A. The proliferation, migration, and invasion abilities of the control and experimental groups were assessed using EDU fluorescence detection, cloning, scratch, and transwell assays. The molecular mechanism underlying ARPC4 regulation by Aurora-A was explored using Western blot analysis. Results: ARPC4 expression was found to decrease in Aurora-A knockdown cells and increase in Aurora-A overexpression cells. Patients with high ARPC4 expression had significantly shorter overall survival compared to those with low expression. Knockdown of ARPC4 counteracted the proliferation of cervical cancer cells induced by Aurora-A overexpression. Migration and invasion capabilities were suppressed in Aurora-A overexpression cell lines following ARPC4 knockdown. Aurora-A activation of the NF-κB p65 signaling pathway led to an upregulation of ARPC4 expression. Conclusion: ARPC4 expression is regulated by Aurora-A, and its knockdown mitigates the effects of Aurora-A overexpression on cervical cancer cells. Aurora-A activation of the NF-κB p65 signaling pathway upregulates ARPC4 expression, providing a potential therapeutic target for cervical cancer treatment. Cervical cancer Aurora-A ARPC4 Proliferation attack transfer Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3884961","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":268745897,"identity":"87f73f16-8da3-4cd5-8194-a1de7fec1cc6","order_by":0,"name":"Yaqing Yue","email":"","orcid":"","institution":"The First Affiliated Hospital Of Shandong Second Medical University, Weifang People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Yaqing","middleName":"","lastName":"Yue","suffix":""},{"id":268745898,"identity":"6d922ed7-5168-46c7-a6af-272afdaae019","order_by":1,"name":"Zhaoxia Mu","email":"","orcid":"","institution":"The First Affiliated Hospital Of Shandong Second Medical University, Weifang People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Zhaoxia","middleName":"","lastName":"Mu","suffix":""},{"id":268745899,"identity":"163d93ee-39d0-4929-8598-ca7f9862a5eb","order_by":2,"name":"Xibo Wang","email":"","orcid":"","institution":"The First Affiliated Hospital Of Shandong Second Medical University, Weifang People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Xibo","middleName":"","lastName":"Wang","suffix":""},{"id":268745900,"identity":"c638f4ef-abe6-47ff-9dd9-f15a595c8725","order_by":3,"name":"Yan Liu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAyUlEQVRIiWNgGAWjYBACPmYwxSbHz97Y+OADMVrYIFr4jCV7DjcbziBKC4SSS9wwI71NmoMoLew8ZhI/d5glbpB82CDNwGAnp9tA0GE8ZpK9Z9KMt0snNhgXMCQbmx0gQosEb9sx2Z2zExuSZzAcSNxGjBbJv23/GTfcPNhwmIdYLdK8bWyKG24wNjYTqYWt2Fq2jQ0YyInNjDMMiPALP//hjTfftoGi8vjzHx8q7OQIagECFgkE24CwchBgJiqZjIJRMApGwQgGAERRPGMUcGGfAAAAAElFTkSuQmCC","orcid":"","institution":"The First Affiliated Hospital Of Shandong Second Medical University, Weifang People's Hospital","correspondingAuthor":true,"prefix":"","firstName":"Yan","middleName":"","lastName":"Liu","suffix":""}],"badges":[],"createdAt":"2024-01-21 14:21:52","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3884961/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3884961/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":50214642,"identity":"bdbcfc30-19ee-421e-b132-9a69777cdb65","added_by":"auto","created_at":"2024-01-26 13:22:37","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":711820,"visible":true,"origin":"","legend":"","description":"","filename":"AuroraARegulatingCervicalCancerInvasionandMetastasisthroughARPC4.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3884961/v1_covered_a53151e0-f45c-420f-9c10-a0f40f3d3c4c.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Aurora-A Regulating Cervical Cancer Invasion and Metastasis through ARPC4","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Cervical cancer, Aurora-A, ARPC4, Proliferation, attack, transfer","lastPublishedDoi":"10.21203/rs.3.rs-3884961/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3884961/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjective：\u003c/strong\u003eTo investigate the impact of ARPC4 knockdown on cervical cancer cells with Aurora-A overexpression in terms of proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods:\u003c/strong\u003eGene expression profiling by RNA-seq, qPCR, and Western blotting were used to identify genes potentially regulated by Aurora-A. The proliferation, migration, and invasion abilities of the control and experimental groups were assessed using EDU fluorescence detection, cloning, scratch, and transwell assays. The molecular mechanism underlying ARPC4 regulation by Aurora-A was explored using Western blot analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003eARPC4 expression was found to decrease in Aurora-A knockdown cells and increase in Aurora-A overexpression cells. Patients with high ARPC4 expression had significantly shorter overall survival compared to those with low expression. Knockdown of ARPC4 counteracted the proliferation of cervical cancer cells induced by Aurora-A overexpression. Migration and invasion capabilities were suppressed in Aurora-A overexpression cell lines following ARPC4 knockdown. Aurora-A activation of the NF-κB p65 signaling pathway led to an upregulation of ARPC4 expression.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003eARPC4 expression is regulated by Aurora-A, and its knockdown mitigates the effects of Aurora-A overexpression on cervical cancer cells. Aurora-A activation of the NF-κB p65 signaling pathway upregulates ARPC4 expression, providing a potential therapeutic target for cervical cancer treatment.\u003c/p\u003e","manuscriptTitle":"Aurora-A Regulating Cervical Cancer Invasion and Metastasis through ARPC4","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-01-25 21:27:29","doi":"10.21203/rs.3.rs-3884961/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"cb5b972d-b51b-485f-a619-6ecb66e35941","owner":[],"postedDate":"January 25th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-02-01T08:29:59+00:00","versionOfRecord":[],"versionCreatedAt":"2024-01-25 21:27:29","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3884961","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3884961","identity":"rs-3884961","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}