Voluntary long-term exercise by female mice modulates anxiety-like behavior and motor function but minimally impacts acute oxidative injury in the central nervous system

Voluntary long-term exercise by female mice modulates anxiety-like behavior and motor function but minimally impacts acute oxidative injury in the central nervous system | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 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Data may be preliminary. 8 July 2025 V1 Latest version Share on Voluntary long-term exercise by female mice modulates anxiety-like behavior and motor function but minimally impacts acute oxidative injury in the central nervous system Author : Yifei Dong 0000-0003-1483-2046 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175194969.94339641/v1 186 views 148 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Physical and cognitive decline from a sedentary lifestyle and aging are detrimental to the health and function of the central nervous system (CNS). As people living in developed societies adopt more sedentary lifestyles with age, identifying cost-efficient strategies to mitigate physical and cognitive decline is critical for improving long-term health care outcomes. While accumulating evidence suggests that moderate aerobic exercise can acutely enhance cognitive decline and improve physical function, the ability of voluntary long-term exercise (VLTE) to improve CNS health and resilience remains less well understood. Here, we assessed how VLTE affected the health and function of the CNS by comparing female mice with access to a functional or disabled running wheel for 6-months. Notably, VLTE limited weight gain in mice and significantly upregulated gene expression in pathways related to synapse function and ion transport in neuroglial cells from the brain. While mice with VLTE had similar short-term memory performance as sedentary mice, VLTE significantly reduced anxiety-like behavior and altered motor function by 6 months. Despite these transcriptomic and behavioral changes, VLTE did not modulate acute oxidative injury induced by oxidized phosphatidylcholine in the spinal cord white matter of mice, suggesting that VLTE may not be sufficient to overcome severe oxidative injury in the CNS. Supplementary Material File (figure 1. weight diff-01.tif) Download 8.59 MB File (figure 2. scrnaseq qc-01.tif) Download 8.41 MB File (figure 3. scrnaseq degs-01.tif) Download 7.58 MB File (figure 4. scrnaseq pathway-01.tif) Download 10.86 MB File (figure 5. nor-01.tif) Download 3.80 MB File (figure 6. oft-01.tif) Download 11.78 MB File (figure 7. spt-01.tif) Download 3.81 MB File (figure 8. lesion-01.tif) Download 43.66 MB File (vlte pilot manuscript 250702.docx) Download 290.25 KB Information & Authors Information Version history V1 Version 1 08 July 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Collection European Journal of Neuroscience Keywords nucleus accumbens object recognition oxidized phosphatidylcholine wheel-running Authors Affiliations Yifei Dong 0000-0003-1483-2046 [email protected] University of Saskatchewan View all articles by this author Metrics & Citations Metrics Article Usage 186 views 148 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Yifei Dong. Voluntary long-term exercise by female mice modulates anxiety-like behavior and motor function but minimally impacts acute oxidative injury in the central nervous system. Authorea . 08 July 2025. DOI: https://doi.org/10.22541/au.175194969.94339641/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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