Effects of Progesterone on Ectopic Tissue Formation in Nude Mice

Objective:To investigate the effect of progesterone on DMEM/Ham's F-12 solution,which contained estrogen,and to assess the functions of progesterone in ectopic tissue formation in nude mice.Methods:The eutopic tissues collected from patients with endometriosis(4 cases)or adenomyosis(1 case)were cultured 24 hours in vitro with estrogen(10 nmol/L,E group)or estrogen plus progesterone(100 nmol/L,E+P group)based on DMEM/Ham's F-12 solution,respectively.After hatching,the tissues were transplanted intraperitoneally into the nude mice.The expression of VEGF,Ki-67 and PR were examined by immunohistochemical analysis before and after 24 h culture in vitro to find the different hormone affection in the two cultural conditions.Results:There was a tendency to increase in number of focus(Z = 2.21,P 0.05)and decrease in diameter of focus(Z = 2.24,P 0.05)in E+P group compared with those of E group.There was a tendency to decrease in expression of PR and Ki-67 in E+P group compared with those of E group.The expression of Ki-67 appeared decreasing in focus of mouse modal(Z = 2.27,P 0.05).The microvessel density was significantly decreased in focus(Z = 2.73,P 0.05).Conclusion:Progesterone(100 nmol/L)cultural condition in vitro may be conducive to forming the ectopic tissue in nude mice but no enhancing for micro blood vessel formation and cytoplasm.