New molecular probes reveal callose structural diversity in plant cell wall microdomains

New molecular probes reveal callose structural diversity in plant cell wall microdomains | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article New molecular probes reveal callose structural diversity in plant cell wall microdomains Yoselin Benitez-Alfonso, Sam Amsbury, Richa Yeshvekar, Susan Marcus, and 14 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8703894/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Cell walls underpin the mechanics of cell growth, intercellular signalling, and defence against pathogenic organisms. β-(1,3)-glucans (also known as callose) are polysaccharides found in plants, fungi, and some bacterial species. In developing plant organs, callose accumulates around intercellular channels (plasmodesmata) controlling cell-to-cell communication. We developed monoclonal antibodies for the detection of β-(1,3)-glucans and using these identified distinct populations of callose differing in size and secondary structure. Callose sub-populations were in proximal but not overlapping cell wall microdomains surrounding plasmodesmata. Moreover, labelling with an antibody that detects larger more complex β-(1,3)-glucans structures (named LM-BDG2) was absent in Arabidopsis meristems implying distinct spatial and functional microenvironments. Biochemical and studies with mutants suggest that the accumulation of LM-BDG2-labelled callose structures is influenced by interactions with xyloglucans (another plant glycan regulating cell wall properties). This work extends current understanding and demonstrates structural heterogeneity in plant β-(1,3)-glucans. It supports interactions between glycans with roles in the regulation of cell wall properties and functions. Biological sciences/Plant sciences/Plant cell biology/Cell wall Biological sciences/Biochemistry/Carbohydrates/Polysaccharides callose β-(1 3)-glucan glycan polymers monoclonal antibodies cell walls polysaccharides xyloglucans plants Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupplementalmaterialsAmsburyetal.pdf Supplementary file BDG13DGmovie.m4v VIDEO S1 BDG23DGmovie.m4v VIDEO S2 bdg2pG1090icals3m002.avi VIDEO S3 Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8703894","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":581279085,"identity":"992f5db0-9e24-4b2e-94b1-9b04ab64cf0f","order_by":0,"name":"Yoselin Benitez-Alfonso","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0001-9779-0413","institution":"The University of Leeds","correspondingAuthor":true,"prefix":"","firstName":"Yoselin","middleName":"","lastName":"Benitez-Alfonso","suffix":""},{"id":581279086,"identity":"7dde4638-bda0-4269-9ad3-35c4e10d430a","order_by":1,"name":"Sam Amsbury","email":"","orcid":"https://orcid.org/0000-0002-2767-9768","institution":"Plants Photosynthesis and Soil, School of Biosciences, The University of Sheffield","correspondingAuthor":false,"prefix":"","firstName":"Sam","middleName":"","lastName":"Amsbury","suffix":""},{"id":581279087,"identity":"9541a178-aef9-492b-8dd5-b6667e212e40","order_by":2,"name":"Richa Yeshvekar","email":"","orcid":"","institution":"University of Leeds","correspondingAuthor":false,"prefix":"","firstName":"Richa","middleName":"","lastName":"Yeshvekar","suffix":""},{"id":581279088,"identity":"b8389738-beee-469c-a989-65a2d66804e9","order_by":3,"name":"Susan Marcus","email":"","orcid":"","institution":"University of Leeds","correspondingAuthor":false,"prefix":"","firstName":"Susan","middleName":"","lastName":"Marcus","suffix":""},{"id":581279089,"identity":"7bb3604a-dd8c-485e-808a-dfdcd517fc45","order_by":4,"name":"Jennifer Barber","email":"","orcid":"","institution":"University of Leeds","correspondingAuthor":false,"prefix":"","firstName":"Jennifer","middleName":"","lastName":"Barber","suffix":""},{"id":581279090,"identity":"d861c1b9-f02a-4aa4-b5d9-094540e67e2a","order_by":5,"name":"Liam German","email":"","orcid":"","institution":"University of Leeds","correspondingAuthor":false,"prefix":"","firstName":"Liam","middleName":"","lastName":"German","suffix":""},{"id":581279091,"identity":"e84653a8-1170-4234-9458-d5497c43d6df","order_by":6,"name":"James Ross","email":"","orcid":"","institution":"University of Leeds","correspondingAuthor":false,"prefix":"","firstName":"James","middleName":"","lastName":"Ross","suffix":""},{"id":581279092,"identity":"cd6f2a3b-3260-4cb3-a0e9-1994667e050f","order_by":7,"name":"Iain Manfield","email":"","orcid":"https://orcid.org/0000-0003-3765-0325","institution":"University of Leeds","correspondingAuthor":false,"prefix":"","firstName":"Iain","middleName":"","lastName":"Manfield","suffix":""},{"id":581279093,"identity":"8e7ddd0e-dc74-4554-bd95-3f7a43886f2e","order_by":8,"name":"J. 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