Iris gatesii foster: Acute toxicity and Hepatoprotective effects of against thioactamid-induced liver Cirrhosis in rats via modulation of PCNS/TGF-β1pathways

Iris gatesii foster: Acute toxicity and Hepatoprotective effects of against thioactamid-induced liver Cirrhosis in rats via modulation of PCNS/TGF-β1pathways | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Iris gatesii foster: Acute toxicity and Hepatoprotective effects of against thioactamid-induced liver Cirrhosis in rats via modulation of PCNS/ TGF - β1 pathways khaled abdul-aziz ahmed, ahmed abduljabbar, mohammed M.Hussein M.Raouf, and 9 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4169345/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Iris gatesii is a well-known therapeutic plant used in Kurdish folk medicine for the treatment of inflammation and tumors. We evaluated the acute toxicity and hepatoprotective effects of methanolic extracts of Iris gatesii (MEIG) against thioacetamide (TAA)-induced liver injury in rats. Albino Dawley rats (30) were clustered into five groups: normal (A) and TAA control rats (B) treated with daily distilled water; reference rats received three oral doses of 50 mg/kg silymarin; D and E, rats received daily doses of 250 and 500 mg/kg MEIG, respectively. In addition, groups B-E received three injections of 200 mg/kg TAA in a week for 60 days. The hepatotoxic results revealed a noticeably lower hepatic injury in MEIG-treated rats, as indicated by the reduced liver index and hepatocyte proliferation. Histopathological evaluation (H&E and Masson trichrome staining) showed significant inhibitory potential of MEIG on the incidence rate of hepatic lesions, represented by decreased liver necrosis and lower fibrous connective tissue proliferation initiated by TAA in rats. MEIG treatment significantly downregulated proliferating cell nuclear antigen (PCNA) in the liver and spleen parenchymal tissues. Moreover, MEIG-treated rats had lower TGF-β1 tissue expression, higher antioxidants (SOD, CAT, and GPx), and reduced MDA content in hepatic tissues compared to cirrhotic control rats. Pro-inflammatory cytokines (IL-10) were negatively modulated, and anti-inflammatory cytokines were noticeably higher in MEIG-treated rats than in cirrhotic control rats. In addition, MEIG treatment significantly restored serum liver biomarkers (enzymes and proteins) in TAA-induced hepatotoxicity. Liver cirrhosis Thioacetamide antioxidant enzymes Histology PCNA TGF-β1 Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4169345","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":574732215,"identity":"651780dd-93c0-4d98-ba5c-664448c191bb","order_by":0,"name":"khaled abdul-aziz ahmed","email":"","orcid":"","institution":"Al-Ahliyya Amman University","correspondingAuthor":false,"prefix":"","firstName":"khaled","middleName":"abdul-aziz","lastName":"ahmed","suffix":""},{"id":574732216,"identity":"bccec3c1-0f46-496c-97bb-a7d1bf5d5582","order_by":1,"name":"ahmed abduljabbar","email":"","orcid":"","institution":"Erbil Technical Health and Medical 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Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Liver cirrhosis, Thioacetamide, antioxidant enzymes, Histology, PCNA, TGF-β1","lastPublishedDoi":"10.21203/rs.3.rs-4169345/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4169345/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cem\u003eIris gatesii\u003c/em\u003e is a well-known therapeutic plant used in Kurdish folk medicine for the treatment of inflammation and tumors.\u003c/p\u003e\n\u003cp\u003eWe evaluated the acute toxicity and hepatoprotective effects of methanolic extracts of Iris gatesii (MEIG) against thioacetamide (TAA)-induced liver injury in rats.\u003c/p\u003e\n\u003cp\u003eAlbino Dawley rats (30) were clustered into five groups: normal (A) and TAA control rats (B) treated with daily distilled water; reference rats received three oral doses of 50 mg/kg silymarin; D and E, rats received daily doses of 250 and 500 mg/kg MEIG, respectively. In addition, groups B-E received three injections of 200 mg/kg TAA in a week for 60 days.\u003c/p\u003e\n\u003cp\u003eThe hepatotoxic results revealed a noticeably lower hepatic injury in MEIG-treated rats, as indicated by the reduced liver index and hepatocyte proliferation. Histopathological evaluation (H\u0026amp;E and Masson trichrome staining) showed significant inhibitory potential of MEIG on the incidence rate of hepatic lesions, represented by decreased liver necrosis and lower fibrous connective tissue proliferation initiated by TAA in rats. MEIG treatment significantly downregulated proliferating cell nuclear antigen (PCNA) in the liver and spleen parenchymal tissues. Moreover, MEIG-treated rats had lower TGF-β1 tissue expression, higher antioxidants (SOD, CAT, and GPx), and reduced MDA content in hepatic tissues compared to cirrhotic control rats. Pro-inflammatory cytokines (IL-10) were negatively modulated, and anti-inflammatory cytokines were noticeably higher in MEIG-treated rats than in cirrhotic control rats. In addition, MEIG treatment significantly restored serum liver biomarkers (enzymes and proteins) in TAA-induced hepatotoxicity.\u003c/p\u003e","manuscriptTitle":"Iris gatesii foster: Acute toxicity and Hepatoprotective effects of against thioactamid-induced liver Cirrhosis in rats via modulation of PCNS/TGF-β1pathways","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-01-16 11:47:34","doi":"10.21203/rs.3.rs-4169345/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a12e575d-371d-45a0-8b75-5537fe703bc9","owner":[],"postedDate":"January 16th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-01-16T11:47:35+00:00","versionOfRecord":[],"versionCreatedAt":"2026-01-16 11:47:34","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4169345","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4169345","identity":"rs-4169345","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}