Upscaling irradiation protocols of Aedes albopictus pupae within an SIT Programme in Reunion Island

Upscaling irradiation protocols of Aedes albopictus pupae within an SIT Programme in Reunion Island | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Upscaling irradiation protocols of Aedes albopictus pupae within an SIT Programme in Reunion Island Lucie Marquereau, Hanano Yamada, David Damiens, Antonin Leclercq, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3829903/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 9 You are reading this latest preprint version Abstract The implementation of the sterile insect technique against Aedes albopictus relies on many parameters, in particular on the success of the sterilization of males, released into the target area in overflooding numbers to mate with wild females. Achieving consistent sterility levels requires efficient and standardized irradiation protocols. Here, we assessed the effects of exposure environment, density of pupae, irradiation dose, quantity of water and location in the canister on the induced sterility of male pupae. We found that irradiation of 2000 pupae in 130 ml of water and with a dose of 40 Gy was the best combination of factors to reliably sterilize male pupae with the specific irradiator used in our control programme, allowing the sterilization of 14000 pupae per cycle. The location in the canister had no effect on induced sterility. The results reported here allowed the standardization and optimization of irradiation protocols for an SIT programme to control Ae. albopictus on Reunion Island, which required the production of more than 300,000 sterile males per week. Biological sciences/Biotechnology Earth and environmental sciences/Ecology Sterile Insect Technique upscale irradiation method Aedes albopictus Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 06 Mar, 2024 Reviews received at journal 14 Feb, 2024 Reviewers agreed at journal 06 Feb, 2024 Reviewers agreed at journal 04 Feb, 2024 Reviewers invited by journal 04 Feb, 2024 Editor assigned by journal 04 Feb, 2024 Editor invited by journal 24 Jan, 2024 Submission checks completed at journal 24 Jan, 2024 First submitted to journal 02 Jan, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3829903","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":269591154,"identity":"5f3acb0b-82d7-4031-823b-ace51429165b","order_by":0,"name":"Lucie Marquereau","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Lucie","middleName":"","lastName":"Marquereau","suffix":""},{"id":269591155,"identity":"5de94333-a2b4-4fd7-b8d7-d507474a7e87","order_by":1,"name":"Hanano Yamada","email":"","orcid":"","institution":"Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, IAEA","correspondingAuthor":false,"prefix":"","firstName":"Hanano","middleName":"","lastName":"Yamada","suffix":""},{"id":269591156,"identity":"7416b4d5-23b8-47b8-9fa3-6129c7a16851","order_by":2,"name":"David Damiens","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"David","middleName":"","lastName":"Damiens","suffix":""},{"id":269591157,"identity":"84280704-6673-4c1c-847d-1c1689ac6c5d","order_by":3,"name":"Antonin Leclercq","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Antonin","middleName":"","lastName":"Leclercq","suffix":""},{"id":269591158,"identity":"ae0543ab-9ffb-4aac-8649-f4e2b9813a45","order_by":4,"name":"Brice Derepas","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Brice","middleName":"","lastName":"Derepas","suffix":""},{"id":269591159,"identity":"1c2e8119-2df5-4ff6-97a1-b42dd806288d","order_by":5,"name":"Cécile Brengues","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Cécile","middleName":"","lastName":"Brengues","suffix":""},{"id":269591161,"identity":"89edd623-5330-47ba-92f2-e166ec895d19","order_by":6,"name":"Brice William Dain","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Brice","middleName":"William","lastName":"Dain","suffix":""},{"id":269591163,"identity":"a79d40fa-0519-425a-9ab6-1c912d9c0d11","order_by":7,"name":"Quentin Lejarre","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Quentin","middleName":"","lastName":"Lejarre","suffix":""},{"id":269591165,"identity":"983de5be-cdda-4d7f-b010-c9f911c82dbb","order_by":8,"name":"Mickael Proudhon","email":"","orcid":"","institution":"UMR MIVEGEC (IRD-CNRS-Université Montpellier), IRD La Réunion – PTU","correspondingAuthor":false,"prefix":"","firstName":"Mickael","middleName":"","lastName":"Proudhon","suffix":""},{"id":269591168,"identity":"112bef4d-7a95-4874-b23e-548833aea4fb","order_by":9,"name":"Jeremy Bouyer","email":"","orcid":"","institution":"UMR ASTRE (CIRAD, INRAE, Université Montpellier)","correspondingAuthor":false,"prefix":"","firstName":"Jeremy","middleName":"","lastName":"Bouyer","suffix":""},{"id":269591169,"identity":"5c5f23b6-bef1-4852-a8db-d395d5bec1a5","order_by":10,"name":"Louis Clément Gouagna","email":"data:image/png;base64,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","orcid":"","institution":"UMR Mivegec (IRD-CNRS-Université Montpellier) - 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