Moxifloxacin hydrochloride loaded proliposomal gel: Formulation, characterization and therapeutic evaluation of periodontitis

Moxifloxacin hydrochloride loaded proliposomal gel: Formulation, characterization and therapeutic evaluation of periodontitis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Moxifloxacin hydrochloride loaded proliposomal gel: Formulation, characterization and therapeutic evaluation of periodontitis Rushikesh Vishwas Lavhate, Rakesh Pandit Dhavale, Dnyaneshwar Mahadev Mane, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6753048/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 16 Feb, 2026 Read the published version in BioNanoScience → Version 1 posted 9 You are reading this latest preprint version Abstract Periodontitis is a common oral disease which cause irreversible damage to periodontal tissues. This study focuses on the development of a Moxifloxacin hydrochloride (MH) -loaded proliposomal gel designed for prolonged retention in the oral cavity to treat periodontal infections. A novel proliposomal gel containing MH-loaded proliposomes prepared by film dispersion freeze drying method. Moxifloxacin hydrochloride was entrapped within the proliposomal vesicles to enhance localised therapeutic efficacy and minimise systemic side effects. The proliposomal gel formulations were evaluated for entrapment efficiency, particle size, zeta potential and morphological studies. The optimized proliposomes were subjected to gel formation, characterised for mucoadhesiveness, pH and drug content, viscosity and rheological behaviour. The optimised batch was biologically evaluated for irritation and antimicrobial studies.The entrapment efficiency ranged from 70.1% to 91.4%, batch PL4 showed the highest efficiency. The optimised formulation demonstrated 215.2 nm particle size, 0.139 PDI, and -21.7 mV zeta potential. TEM analysis confirmed the formation of spherical bilayer vesicles. The PLG showed good mucoadhesiveness, pH compatibility and drug content. Rheological studies indicated optimal viscosity for prolonged oral retention, and in-vitro release studies showed sustained drug release over 12 hours. Ex-vivo permeation studies revealed improved drug delivery compared to a conventional gel. The formulation exhibited significant antibacterial activity against Staphylococcus aureus and Escherichia coli . and HET CAM showed no signs of irritation, confirming its safety. Thus, Moxifloxacin loaded proliposomal gel (PLG) may offer an effective, stable, and sustained release drug delivery system, enhancing therapeutic outcomes in periodontitis treatment and improving patient compliance. HET-CAM study Moxifloxacin HCL Periodontitis Proliposomal gel Proliposome Full Text Additional Declarations No competing interests reported. Supplementary Files Supplimentaryinformation.docx Cite Share Download PDF Status: Published Journal Publication published 16 Feb, 2026 Read the published version in BioNanoScience → Version 1 posted Editorial decision: Revision requested 19 Aug, 2025 Reviews received at journal 18 Aug, 2025 Reviewers agreed at journal 01 Aug, 2025 Reviews received at journal 01 Aug, 2025 Reviewers agreed at journal 17 Jul, 2025 Reviewers invited by journal 17 Jul, 2025 Editor assigned by journal 05 Jun, 2025 Submission checks completed at journal 05 Jun, 2025 First submitted to journal 26 May, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6753048","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":488011047,"identity":"b9a39e23-2a64-4cd7-9e9a-85b63d3aa0d2","order_by":0,"name":"Rushikesh Vishwas Lavhate","email":"","orcid":"","institution":"Bharati Vidyapeeth College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Rushikesh","middleName":"Vishwas","lastName":"Lavhate","suffix":""},{"id":488011050,"identity":"f6dd3f00-82c0-441f-8c21-b54dc255f4f1","order_by":1,"name":"Rakesh Pandit 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This study focuses on the development of a Moxifloxacin hydrochloride (MH) -loaded proliposomal gel designed for prolonged retention in the oral cavity to treat periodontal infections. A novel proliposomal gel containing MH-loaded proliposomes prepared by film dispersion freeze drying method. Moxifloxacin hydrochloride was entrapped within the proliposomal vesicles to enhance localised therapeutic efficacy and minimise systemic side effects. The proliposomal gel formulations were evaluated for entrapment efficiency, particle size, zeta potential and morphological studies. The optimized proliposomes were subjected to gel formation, characterised for mucoadhesiveness, pH and drug content, viscosity and rheological behaviour. The optimised batch was biologically evaluated for irritation and antimicrobial studies.The entrapment efficiency ranged from 70.1% to 91.4%, batch PL4 showed the highest efficiency. 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