New defined and semi-defined media for cultivation of the flagellate Trichomonas vaginalis

D. Linstead
In: Parasitology · 1981 · vol. 83(1) , pp. 125–137 · doi:10.1017/s0031182000050101 · PMID:6973740 · W2168646896
article OA: closed CC0
View on OpenAlex View on PubMed View at publisher

Abstract

Studies on the nutrition and physiology of Trichomonas vaginalis have been hampered by the lack of a defined synthetic medium in which to grow the organism. New synthetic media free of ill-defined supplements and serum which support good growth of T. vaginalis have been developed in this study. The first successful approach to a defined medium resulted from the modification of the tissue-culture medium CMRL 1066. Essential modifications included (1) lowering the redox potential of the medium by adding high levels of ascorbate and (2) modifying the buffer system. Subsequently, CMRL 1066 was replaced by two new media DL7 and DL8 which have greater flexibility in their formulation and permit improved serum-free growth. Serum was replaced by bovine serum albumin and cholesterol, together with either a glyceryl fatty acid ester or a defined mixture of fatty acids. Preliminary nutritional studies using the new media have indicated a requirement for saturated and unsaturated fatty acids and a sterol.

My notes (saved in your browser only)

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.

References (18)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK