Real-Time Observations of Leaf Vitality Extinction by Dynamic Speckle Imaging

Real-Time Observations of Leaf Vitality Extinction by Dynamic Speckle Imaging | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Real-Time Observations of Leaf Vitality Extinction by Dynamic Speckle Imaging Elise COLIN, Enrique Garcia Caurel, Karine Adeline, Aurélien Plyer, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4777448/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 18 Nov, 2024 Read the published version in Photonics → Version 1 posted You are reading this latest preprint version Abstract Sap flow within a leaf is a critical indicator of plant vitality and health. This paper introduces an easy-to-use, non-invasive and real-time imaging method for sap microcirculation imaging. From the coherent backscattering of light on a leaf, we show that the acquisition frequency of dynamic speckle can be linked to the microcirculation speed inside the leaf. Thus, by accessing different ranges of sap flow velocities, we reveal in a non invasive way, the anatomy of the leaf's circulatory network with unprecedented richness. We experimentally validate the ability of the method to characterize in real time the vitality of a fig leaf by observing the continuous decrease of sap circulation, first in the smaller vessels and then in the larger ones, following the cutting of the leaf over a 48-hour period. Physical sciences/Optics and photonics/Applied optics/Optical sensors Physical sciences/Physics/Electronics, photonics and device physics/Photonic devices dynamic speckle time-series leaf sap Fujii index Full Text Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Published Journal Publication published 18 Nov, 2024 Read the published version in Photonics → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4777448","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":331193831,"identity":"7c8932fb-3390-4b88-9129-75b5d1419b7b","order_by":0,"name":"Elise COLIN","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA3klEQVRIiWNgGAWjYDACdsYGMM3HzMD4ACIEo3EBZqgWNmYGZgOokAEBLVCaDYgkiNLCz8zc/OEDg50cGzvvsYqPO2zy+RuY2T7g0yLZzNgmOYMh2ZiNmS/t5swzaZYzDjAzz8CnxeAwYxszD8OBRCBpdpu37bABwwH+w3gdZn+YsfnzH6iWYt62/wbyQFvwajEAhpg0A1QLM2/bAQMDQlokgA6T7DEA+YXHWHJmW7KB4WECWvjb2x9/+FFhJ8fPf8bww8c2OwO54834tUCdh8whRsMoGAWjYBSMAvwAAPzUOKkQcybpAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-7401-8073","institution":"ONERA","correspondingAuthor":true,"prefix":"","firstName":"Elise","middleName":"","lastName":"COLIN","suffix":""},{"id":331193832,"identity":"f807d389-e313-418a-a9eb-ae8195b1bec8","order_by":1,"name":"Enrique Garcia Caurel","email":"","orcid":"","institution":"École Polytechnique","correspondingAuthor":false,"prefix":"","firstName":"Enrique","middleName":"Garcia","lastName":"Caurel","suffix":""},{"id":331193833,"identity":"5894c16d-6ab6-42c4-9068-068efe81af7f","order_by":2,"name":"Karine Adeline","email":"","orcid":"","institution":"ONERA","correspondingAuthor":false,"prefix":"","firstName":"Karine","middleName":"","lastName":"Adeline","suffix":""},{"id":331193834,"identity":"988df07f-30a9-454d-ac9d-8994a0ec94e6","order_by":3,"name":"Aurélien Plyer","email":"","orcid":"","institution":"ONERA","correspondingAuthor":false,"prefix":"","firstName":"Aurélien","middleName":"","lastName":"Plyer","suffix":""},{"id":331193835,"identity":"940d519c-3306-4bc6-9254-befd95ec3816","order_by":4,"name":"Xavier Orlik","email":"","orcid":"","institution":"ONERA","correspondingAuthor":false,"prefix":"","firstName":"Xavier","middleName":"","lastName":"Orlik","suffix":""}],"badges":[],"createdAt":"2024-07-21 15:45:30","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-4777448/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4777448/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.3390/photonics11111086","type":"published","date":"2024-11-19T00:00:00+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":69459128,"identity":"b43c70a6-32f2-4549-b26f-1440ff5fb6e2","added_by":"auto","created_at":"2024-11-20 14:30:36","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1133881,"visible":true,"origin":"","legend":"Article File","description":"","filename":"FujiiLeaf3.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4777448/v1_covered_d6791c5f-dbac-41f7-a67e-b88fffa465c6.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Real-Time Observations of Leaf Vitality Extinction by Dynamic Speckle Imaging","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"dynamic speckle, time-series, leaf sap, Fujii index","lastPublishedDoi":"10.21203/rs.3.rs-4777448/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4777448/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Sap flow within a leaf is a critical indicator of plant vitality and health. 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