Characterization of Mild Delayed Gestational Hypertension in Rats Following Ozone Exposure

Characterization of Mild Delayed Gestational Hypertension in Rats Following Ozone Exposure | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Characterization of Mild Delayed Gestational Hypertension in Rats Following Ozone Exposure Russell Hunter, Thomas Wilson, Selita Lucas, David Scieszka, Barry Bleske, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3977101/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 9 You are reading this latest preprint version Abstract The contribution of air pollution induced cardio-pulmonary damage on the development of hypertensive disorders of pregnancy and other adverse outcomes of pregnancy has gained increased attention as epidemiological data continues to highlight spatiotemporal pregnancy trends related to air pollution exposure. However clinical mechanistic data surrounding gestational complications remains sparse, necessitating the need for the use of animal models to study these types of complications of pregnancy. The current study seeks to examine the real-time effects of mid-gestational ozone exposure on maternal blood pressure and body temperature through the use of radiotelemetry in a rat model. The exposure resulted in acute depression of heart rate and core body temperature as compared to control animals. Ozone exposed animals also presented with a slight but significant increase in arterial blood pressure which was perpetuated until term. The data presented here illustrates the feasibility of murine models to assess cardiovascular complications caused by inhaled toxicants during the window of pregnancy. Ozone Air Pollution Pregnancy Hypertension Figures Figure 1 Figure 2 Figure 3 Figure 4 Introduction Air pollution has a known impact on vascular function, driving an increased incidence of inflammatory vascular diseases and hypertension [ 1 ]. Recent studies have identified a potential link between ambient air pollution and incidence of gestational hypertension or preeclampsia, showing positive associations with ambient ozone (O 3 ), nitrogen dioxide, and particulate matter [ 2 – 5 ]. Importantly, using time-series statistical analyses, epidemiological studies highlight that the vulnerable window for this relationship appears to be in early pregnancy [ 5 , 6 ], potentially during placentation and early development of the placenta. These associations remain incompletely characterized in terms of biological plausibility and pathogenesis, with very few toxicological studies conducted to specifically address pollutant-induced gestational hypertension [ 7 ]. Notably, epidemiologically-observed blood pressure changes in late-stage pregnancy have been most clearly aligned with first trimester exposures to air pollution [ 8 ]. Controlled exposures to O 3 promote uterine and placental vascular responses in preclinical models and help explain the biological plausibility of epidemiological assosications. The Kodavanti lab identified uterine artery pathophysiological changes associated with early gestational exposure to 0.4 and 0.8 ppm O 3 . Exposure at implantation (GD5.5) led to small but significant increases in the uterine artery resistance and pulsatile indices at GD21 [ 7 ]. In recent studies of inhaled O 3 in pregnant rats, we have observed substantial serum elevations of soluble Fms-like tyrosine kinase-1 (aka sFlt-1) [ 9 ], which is an essential player in both preeclampsia and peripartum cardiomyopathy [ 10 – 12 ]. Proteomic and phosphoproteomic changes in the cardiac tissue, typified by cardiomyopathic ontological classifications, from pregnant rats exposed to O 3 at GD10.5 were dramatically greater than the changes seen in dams exposed at GD20.5, suggesting that an early impact on placental growth and development may be central to late stage changes.[ 9 ] Furthermore, we have observed significant alterations of uterine vascular physiology and placental transcription products with late-stage O 3 exposure[ 13 ]. Thus, recent preclinical research does indicate a path to gestational hypertension from early- or mid-gestational exposure to air pollution. A missing component of the preclinical investigations into early gestational impacts of air pollution exposure on maternal cardiovascular health is a clear indicator of systemic hypertension, or blood pressure change, especially as develops in late pregnancy and resolves upon delivery (and removal of the offending placenta). In the present study, we therefore sought to assess O 3 exposure effects on blood pressure and related parameters using radiotelemetry for continuous measurement with minimal handling of the pregnant rats. Materials and Methods Animals Female Sprague-Dawley rats were used in this study. Animals were housed in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-approved facility at the University of New Mexico Health Sciences Center and maintained at recommended temperature (20–24°C), relative humidity (30–60%), and on a 12-h light/dark cycle throughout the study. Animals were provided with standard chow and water ad libitum. All animal procedures were conducted humanely and approved by the Institutional Animal Care and Use Committee at the University of New Mexico. Radiotelemetry Implantation Rats were implanted with radiotelemetry devices for continuous acquisition of systemic arterial blood pressure, heart rate (HR), core body temperature (T CO ), and physical activity (PA; HD-S10; DataSciences, Intl; St Paul, MN). Implantation was conducted as previously described. Briefly, under isoflurane anesthesia, the pressure catheter of the telemetry device was advanced through the insertion point of the femoral artery to a position within the abdominal aorta and secured with suture and tissue glue. The body of the telemeter was secured subcutaneously in the flank of the rat. Buprenorphine (0.03 mg/kg subcutaneous) was administered 30 min before the end of surgery and postoperatively as needed to provide analgesia. Rats recovered a minimum of 7 days prior to further experimentation. Mating Once rats recovered from surgery, the fidelity of the blood pressure signal was confirmed (a minimum pulse pressure of 10 mmHg was required). Rats with viable blood pressure signals were then paired with male rats for up to 3 days, with confirmation of successful copulation as evidenced bu the presences of a seminal plug. Following successful plug check or after three days of pairing, female rats were removed and returned to normal housing conditions. Body weights were assessed at regular intervals following this pairing. A > 5% increase in body weights at GD8.5 was consistent with pregnancy (Fig. 1 ). Pregnant rats were then exposed to O3 at mid gestation on GD10.5 (described below). Non-pregnant rats were considered for re-pairing, assuming the radiotelemetry signal remained strong. Three experiments were undertaken to obtain an N = 7 pregnant rats per exposure group with radiotelemetry signals of sufficient quality. Following exposures, rats were followed to term and delivery. O 3 Exposure Dams were exposed to either filtered air (FA) or 1ppm O 3 for a single 4-h period via whole body inhalation. O 3 was generated using an OREC silent arc discharge generator (Osmonics, Phoenix, Arizona) as previously described [ 9 , 14 ]. Water was available for animals during the exposure, but food was withheld. Exposures took place in standard shoebox cages without bedding (to prevent O 3 scavenging). O 3 concentrations were monitored in real-time using an indoor air quality monitor (GrayWolf Sensing Solutions, Shelton, CT) and adjustments to flow were conducted manually to ensure that concentrations were consistently between 0.9–1.1 ppm. Data Analysis and Statistics Data from all rats with sufficient signal quality was included (namely, pulse pressure > 10 mmHg). Data for mean arterial blood pressure (MAP), HR, T CO , and PA were collected every 10 minutes, but averaged to daily values for statistical testing. FA and O 3 groups were compared by a 2-way repeated measures ANOVA to account for time and exposure, with Šídák's multiple comparisons test used to confirm O3 effects at specific time points (GraphPad Prism, v9.5.1). Data are presented as mean +/- SEM; p-values < 0.05 were considered to be significant. Results and Conclusions: Animal Weights Animals were weighed weekly to assess differences in weight gain and as a measure of overall health. Following pairing, female rats were weighed every 2–4 days to assess likelihood of pregnancy (Fig. 1 ). Growth greater than 5% at GD8.5 allowed for a clear demarcation of pregnancy, at which dams were randomized for exposure conditions (FA or O 3 ). Mean arterial blood pressure Evidence of a late-gestational increased in blood pressure was determined in O 3 -exposed rats (Fig. 2 ). Prior to exposures, the FA and O 3 groups, despite randomization, displayed an approximately 3.5 mmHg difference in mean arterial pressure, possibly real or due to calibration variance in the radiotelemetry devices. Regardless, following O 3 exposure at GD10.5, this difference became augmented by approximately 5.0 mmHg from approximately GD15.5 until delivery. To better ascertain the time frame of the increased mean arterial pressure, we normalized values to the period from GD3.5 to GD8.5, when paired mating was over but prior to exposures. With this approach, we revealed significant differences between FA and O 3 -exposed groups from GD15.5 until GD19.5. Notably, deliveries began between GD20.5 and GD22.5, at which point mean arterial pressure differences between exposure groups subsided. PA, HR, and T CO The three additional parameters of PA, HR, and T CO all behaved differently compared to mean arterial pressure (Fig. 3 ). PA was generally not altered as a result of the O 3 exposure (Fig. 3 A). Activity was elevated during pairing with male rats, and reduced for approximately 24h following the O 3 or sham exposure. Moreover, there was a steady reduction in PA throughout the course of pregnancy. Even with removing the first 4 days of data to eliminate effects of pairing with male rats, Time as a factor in the 2-way repeated measures ANOVA was significant (p = 0.0013) while O 3 was not. HR was acutely reduced by the O 3 exposure, on average 43 beats per minute lower than FA-exposed dams and fully resolving 48-72h post exposure (Fig. 3 B). During the period of elevated mean arterial pressure (GD15.5-GD19.5), however, HR values were similar between exposure groups. This acute reduction in HR is consistent with numerous previous studies [ 15 , 16 ]. Interestingly, HR increased in both groups peri-delivery, despite no increase in PA. T CO was consistent between both groups prior to exposure, but displayed an interesting reduction late in pregnancy, significant on GD17.5 (Fig. 3 C). This aligns well with the general period of elevated mean arterial blood pressure (Fig. 2 B and C). Previous research has routinely described an acute hypothermia in mice and rats associated with O 3 exposure [ 16 ], but this delayed response appears unique to the pregnant rat. This acute phenomenon of hypothermia could be observed in the present study if data was viewed more closely (Fig. 3 D). The drop in T CO was substantial (mean = 33.2°C), but resolved within approximately 2h after the O 3 exposure ended. Discussion The use of radiotelemetry in a Sprague Dawley rat model of pregnancy has been employed in several studies to evaluate changes in blood pressure, ostensibly to mimic gestational hypertension and/or preeclampsia. Infusion of the vasoconstrictor angiotensin II on gestational days 6, 9, 12, and 15 has been used to increase mean arterial blood pressure about 20 + mmHg over controls, while maintaining the integrity of the pregnancy [ 17 ]. Severe hypertensive disorders of pregnancy, such as preeclampsia, have also been modeled utilizing intraperitoneal injection of the angiogenesis inhibitor Suramin to increase (17 mmHg) blood pressure [ 18 ]. To our knowledge, this is the first study utilizing radiotelemetry to continuously assess blood pressure of unrestrained pregnant rats in response to an inhaled toxicant such as ozone. Previous studies have examined blood pressure intermittently following ozone exposure in pregnant rats via tail cuff photoplethysmograpy and saw no blood pressure changes [ 7 ], although slight differences in O 3 concentration as well as exposure timing complicate comparing the two studies. O 3 exposure occurring during the initial phases of placentation (GD10.5) led to a delayed elevation of blood pressure in pregnant rats several days following the exposure. While the effect was mild (only a 5 mmHg increase), it was sustained until delivery, consistent with human gestational hypertension, i.e., elevated blood pressure of placental origin. The blood pressure changes were accompanied by a modest reduction in T CO , which may indicate a general morbidity associated with the late stage condition. Changes acutely caused by O 3 exposure, namely bradycardia and hypothermia, are typical of such exposures and rapidly resolved, as previously reported.[ 15 , 16 , 19 ] For reasons described below, the delayed pressor effect of O 3 in the present study is likely a unique pathology elicited by indirect effects on placental growth, setting the stage for a syndrome akin to gestational hypertension in late stages of pregnancy. Mothers are highly vulnerable to pathological cardiovascular and hemodynamic alterations during pregnancy, as the growing fetus and palcenta demand a substantive increase in cardiopulmonary output over the 9 months of gestation [ 20 ]. Under normal circumstances, maternal vascular resistance drops approximately 30% to accommodate increased demands on cardiac output from the growing fetus and the typical mild anemia that occurs. However, environmental factors may interfere with the normal vascular remodeling required for this reduction in systemic resistance, promoting hypertension and cardiomyopathy. Placental insufficiency is linked to maternal hypertension and preeclampsia. Preeclampsia occurs in roughly 3–8% of pregnancies, reflecting a large population at risk, but numerous factors contribute to the etiology.[ 21 ] Incomplete angiogenesis during placentation can lead to a poorly perfused placenta, which responds by releasing proangiogenic and proinflammatory factors.[ 22 ] Maternal systemic blood pressure surges dramatically in preeclampsia, accompanied by proteinuria and elevations in several key circulating factors such as the soluble fms-like tyrosine kinase-1.[ 23 ] In addition to acute risk of seizure, death, or pre-term delivery resulting from a hypertensive crisis, long-term cardiomyopathic, hemodynamic, and cognitive deficits have been observed in preeclampsia patients post-delivery.[ 24 , 25 ] Preeclampsia incidence has a heterogeneous etiology and is influenced in part by genetics, immunology, nutritional status, and environmental exposures,[ 26 – 30 ] but no single factor has a strong predictive role. The present study and other recent research may provide an integrated mechanistic understanding of how air pollution may adversely impact placental development, promoting preeclampsia and related maternal cardiovascular pathologies. As an important component of the mechanisms by which inhaled pollutants impact the systemic vasculature, we have identified that fragmented peptides arising from pulmonary peptidase activity in response to pollutants [ 31 ] can directly stimulate endothelial inflammatory responses in coronary, mesenteric, and cerebrovascular beds [ 32 – 34 ], and there is little reason to assume that the placental vascular bed is not similarly vulnerable to the systemic effects of inhaled toxicants. As a specific example, a fragmented thrombospondin-1 peptide (corresponding to amino acids 402–460) was highly upregulated in serum after pulmonary particulate matter exposure and was observed to reduce endothelial cell growth rates, most likely via interaction with the CD36 receptor.[ 34 , 35 ] We postulate that such mechanisms may directly retard early placental growth especially of the vasculature. Later in gestation, the delayed placental growth may lead to release of pressors like sFlt-1 to direct greater blood flow to the placenta, elevating maternal vascular resistance and blood pressure (Fig. 4 ). One limitation to this study design is that blood pressure response to O 3 was not assessed in non-pregnant rats. Notably several studies have investigated this with limited indication that blood pressure or hemodynamics, in general, are impacted by O 3 . Numerous studies even point to a reduction in blood pressure in vulnerable or two-hit models designed to evince hypertension.[ 36 – 40 ] Normally, O 3 is rapidly scavenged in the epithelial lining fluid of the lung and inflammatory/toxic effects are typically acute and resolved within 24–72 hours, depending on the exposure concentration and extent of lung injury.[ 41 – 43 ] The concentration used in the present study (1 ppm) typically resolves in 24h. Thus, the observation of significant mean arterial pressure changes, along with T CO changes, arising 5–10 days after the exposure suggests there is a unique response in the setting of pregnancy that may promote the pathology of gestational hypertension. Early O 3 -induced deficits of placental growth and development might therefore set the stage for increased uterine artery resistance and ultimately higher blood pressure as demands of the fetus and placenta increase in late pregnancy. A second limitation to this study is the rat model where the differences in gestational timing, litter size, and possibly placental physiology may limit translation to human gestation. However, the differences may also explain why the gestational hypertension seen in the present study was only a modest 5 mmHg, while human preeclampsia is characterized blood pressures exceeding 200 mmHg, i.e ., to life-threatening levels. The perfusion of the rodent uterus may become limited by the O 3 effect on uterine vascular resistance, but the rodent can reabsorb fetuses to balance this outcome and ensure a healthy supply of blood to all progeny, which humans are unable to do. Furthermore, the short duration of gestation (22 days) may simply not be sufficient to generate a profound hypertensive phenotype. Thus, we suspect the mild cardiovascular response in the rodent model implies that increasing the severity of O 3 exposure would lead to spontaneous abortion. In summary, pregnant rats exposed to O 3 at GD10.5 displayed a mild but significant increase in mean arterial blood pressure beginning 5 days after exposure and lasting until term. While this model has limitations and the scope of pathological assessment does not permit a stronger conclusion related to gestational hypertension or preeclampsia, the phenomenon of late-term elevated blood pressure that resolves after delivery is consistent with the debilitating and life-threatening human syndrome of preeclampsia. Integrated within the context of several recent research communications, this adds meaningfully to the understanding of how ambient air pollution may promote the risk of adverse pregnancy outcomes like gestational hypertension or spontaneous abortion. Much research is needed to better delineate the link between pulmonary exposures and placental effects as well as the relative contribution of summated pollutants, diet, genetics and lifestyle that may further contribute to risk of adverse outcomes. Declarations The authors declare they have no financial conflicts of interest with the content of this manuscript. Author Contribution All authors read and approved the manuscript.Major writing was conduct by RH, NLK, BB, and MC.Surgical procedures were conducted by CP, TW, and RHExposures were conducted by SLData collection and analysis was conducted by RH, DL, and MCStudy design and conceptualization was conducted by TW, RH, AO, MC, RA, and NLK Acknowledgements: This reserch was funded by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (F31 HD107945), National Institute of Environmental Health Sciences (R01 ES014639) and the National Institute for General Medical Sciences (P20 GM130422). 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Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 19 May, 2024 Reviews received at journal 19 May, 2024 Reviewers agreed at journal 15 Apr, 2024 Reviews received at journal 08 Mar, 2024 Reviewers agreed at journal 28 Feb, 2024 Reviewers invited by journal 28 Feb, 2024 Editor assigned by journal 27 Feb, 2024 Submission checks completed at journal 21 Feb, 2024 First submitted to journal 21 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3977101","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":274219231,"identity":"4ed9c699-e374-4d4a-96ec-c078cd9b576f","order_by":0,"name":"Russell Hunter","email":"","orcid":"","institution":"University of New Mexico College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Russell","middleName":"","lastName":"Hunter","suffix":""},{"id":274219232,"identity":"dc043bf0-aa01-4b59-8704-a11b91a13429","order_by":1,"name":"Thomas Wilson","email":"","orcid":"","institution":"University of New Mexico College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Thomas","middleName":"","lastName":"Wilson","suffix":""},{"id":274219233,"identity":"ff1a9bbb-dbf4-4aa7-b4df-66528dd147d9","order_by":2,"name":"Selita Lucas","email":"","orcid":"","institution":"University of New Mexico College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Selita","middleName":"","lastName":"Lucas","suffix":""},{"id":274219234,"identity":"86f1074f-14c4-479b-b43e-3feb267ab66e","order_by":3,"name":"David Scieszka","email":"","orcid":"","institution":"University of New Mexico College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"David","middleName":"","lastName":"Scieszka","suffix":""},{"id":274219235,"identity":"008c45ea-7b89-46d1-ab21-d3866d47441f","order_by":4,"name":"Barry Bleske","email":"","orcid":"","institution":"University of New Mexico College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Barry","middleName":"","lastName":"Bleske","suffix":""},{"id":274219236,"identity":"a815829b-7cb0-4567-ae65-7cf08cd52c98","order_by":5,"name":"Andrew Ottens","email":"","orcid":"","institution":"Virginia Commonwealth University","correspondingAuthor":false,"prefix":"","firstName":"Andrew","middleName":"","lastName":"Ottens","suffix":""},{"id":274219237,"identity":"617df4de-1204-4ebd-be49-061273e946f5","order_by":6,"name":"Ryan Ashley","email":"","orcid":"","institution":"New Mexico State University","correspondingAuthor":false,"prefix":"","firstName":"Ryan","middleName":"","lastName":"Ashley","suffix":""},{"id":274219238,"identity":"04319dde-d8ea-4eae-b21f-7d350df0a4a4","order_by":7,"name":"Carolyn Pace","email":"","orcid":"","institution":"University of New Mexico School of Medicine","correspondingAuthor":false,"prefix":"","firstName":"Carolyn","middleName":"","lastName":"Pace","suffix":""},{"id":274219239,"identity":"4746c99c-abe8-4e2d-b37d-78138b0a2bdf","order_by":8,"name":"Nancy Kanagy","email":"","orcid":"","institution":"University of New Mexico School of Medicine","correspondingAuthor":false,"prefix":"","firstName":"Nancy","middleName":"","lastName":"Kanagy","suffix":""},{"id":274219240,"identity":"bdde3ca5-ce5d-492e-969e-d7b9004f1acf","order_by":9,"name":"Matthew J Campen","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA3UlEQVRIiWNgGAWjYBADOQYG5gYGHihPghgtxgwMjCRqSWwgWotu++Gnmwsq7qTPbz/YJvGG4XCebgPzwds8eLSYnUkzuz3jzLPcDWcS2yTnMBwuNjvAlmyNV8sNBrPbvG2HczcwJLZJ8zAcTtx2gMdMGr8W9m+3ef8dTpfvfwjTwv+NgBYeoC0NhxMYbiBsYcOv5UxO2e0Zxw4bbrjxsNlyjkF64rbDbMaWc/BpOX582+2CmsPy8v3JB2+8qbBO3Ha8+eGNN3i0gAAzgmmAyiVGyygYBaNgFIwCLAAANfVRzLZEfq8AAAAASUVORK5CYII=","orcid":"","institution":"University of New Mexico College of Pharmacy","correspondingAuthor":true,"prefix":"","firstName":"Matthew","middleName":"J","lastName":"Campen","suffix":""}],"badges":[],"createdAt":"2024-02-21 23:53:14","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3977101/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3977101/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":51543874,"identity":"deec01b6-1f2a-41d4-b108-2d9f93d2ff98","added_by":"auto","created_at":"2024-02-23 12:17:27","extension":"jpeg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":222358,"visible":true,"origin":"","legend":"\u003cp\u003eNormalized body weight trends for telemetered rats (third experiment shown) following pairing with male rats.\u0026nbsp; By GD8.5 sufficient discrimination was evident to select and randomize rats for exposures.\u0026nbsp; No differences were seen with O\u003csub\u003e3\u003c/sub\u003e exposure\u003c/p\u003e","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3977101/v1/566071a65579a0fcf3027a0e.jpeg"},{"id":51543871,"identity":"008cac50-a665-4b4b-b68e-f99afacb6acc","added_by":"auto","created_at":"2024-02-23 12:17:27","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":15225,"visible":true,"origin":"","legend":"\u003cp\u003eMean arterial blood pressure from pregnant rats (n=7/grp) exposed to filtered air (control) or 1 ppm O\u003csub\u003e3\u003c/sub\u003e for 4h on GD10.5. Shown are (A) raw data (10 minute collections, averaged by group); (B) 24h average data; and (C) data normalized to average readings from GD3.5-GD8.5, a period where handling was minimized and pairing with male rats was complete.\u0026nbsp; Data normalization in (C) was conducted to offset initial group differences apparent in the raw data (A and B).\u0026nbsp; Asterisks indicate significant difference between the FA and O\u003csub\u003e3\u003c/sub\u003e groups, based on a 2-way repeated measures ANOVA with Šídák's post-hoc testing (*p\u0026lt;0.05; **p\u0026lt;0.01; ***p\u0026lt;0.001) using GraphPad Prism\u0026nbsp;\u003c/p\u003e","description":"","filename":"Onlinefloatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-3977101/v1/e1459d3499adfe9e4bfa2b81.png"},{"id":51543872,"identity":"d2e5150d-23d4-4c55-bb6a-3429babb49e0","added_by":"auto","created_at":"2024-02-23 12:17:27","extension":"jpeg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":750153,"visible":true,"origin":"","legend":"\u003cp\u003ePhysical activity (A), heart rate (B), core body temperature (C) for the full 22 day period of pregnancy, and more detailed resolution of core body temperature relative to exposure (D) from pregnant rats (n=7/grp) exposed to filtered air (control) or 1 ppm O\u003csub\u003e3\u003c/sub\u003e for 4h on GD10.5. Asterisks indicate significant difference between the FA and O\u003csub\u003e3\u003c/sub\u003e groups, based on a 2-way repeated measures ANOVA with Šídák's post-hoc testing (*p\u0026lt;0.05) using GraphPad Prism\u0026nbsp;\u003c/p\u003e","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3977101/v1/e2fdeff77bc4d3bc25456d39.jpeg"},{"id":51543873,"identity":"1ca9e2dc-55cb-4e47-9fff-0565a2de735c","added_by":"auto","created_at":"2024-02-23 12:17:27","extension":"jpeg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":591141,"visible":true,"origin":"","legend":"\u003cp\u003eSummarizing studies from our laboratory and others, we propose that inhaled pollutants such as ozone can promote or exacerbate gestational by causing circulating factors arising from the lung that retard placental angiogenesis, which ultimately leads to placental insufficiency later in pregnancy. Figure created using Biorender.\u003c/p\u003e","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3977101/v1/04386e52a11173fb93d963b9.jpeg"},{"id":51544125,"identity":"dc59175a-d2da-4d63-9df1-3ea65cb004b6","added_by":"auto","created_at":"2024-02-23 12:25:27","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":536065,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3977101/v1/df096321-ba6d-40eb-83c7-aeb55b6779bc.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Characterization of Mild Delayed Gestational Hypertension in Rats Following Ozone Exposure","fulltext":[{"header":"Introduction","content":"\u003cp\u003eAir pollution has a known impact on vascular function, driving an increased incidence of inflammatory vascular diseases and hypertension [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. Recent studies have identified a potential link between ambient air pollution and incidence of gestational hypertension or preeclampsia, showing positive associations with ambient ozone (O\u003csub\u003e3\u003c/sub\u003e), nitrogen dioxide, and particulate matter [\u003cspan additionalcitationids=\"CR3 CR4\" citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Importantly, using time-series statistical analyses, epidemiological studies highlight that the vulnerable window for this relationship appears to be in early pregnancy [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e, \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e], potentially during placentation and early development of the placenta. These associations remain incompletely characterized in terms of biological plausibility and pathogenesis, with very few toxicological studies conducted to specifically address pollutant-induced gestational hypertension [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Notably, epidemiologically-observed blood pressure changes in late-stage pregnancy have been most clearly aligned with first trimester exposures to air pollution [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eControlled exposures to O\u003csub\u003e3\u003c/sub\u003e promote uterine and placental vascular responses in preclinical models and help explain the biological plausibility of epidemiological assosications. The Kodavanti lab identified uterine artery pathophysiological changes associated with early gestational exposure to 0.4 and 0.8 ppm O\u003csub\u003e3\u003c/sub\u003e. Exposure at implantation (GD5.5) led to small but significant increases in the uterine artery resistance and pulsatile indices at GD21 [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. In recent studies of inhaled O\u003csub\u003e3\u003c/sub\u003e in pregnant rats, we have observed substantial serum elevations of soluble Fms-like tyrosine kinase-1 (aka sFlt-1) [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e], which is an essential player in both preeclampsia and peripartum cardiomyopathy [\u003cspan additionalcitationids=\"CR11\" citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e]. Proteomic and phosphoproteomic changes in the cardiac tissue, typified by cardiomyopathic ontological classifications, from pregnant rats exposed to O\u003csub\u003e3\u003c/sub\u003e at GD10.5 were dramatically greater than the changes seen in dams exposed at GD20.5, suggesting that an early impact on placental growth and development may be central to late stage changes.[\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e] Furthermore, we have observed significant alterations of uterine vascular physiology and placental transcription products with late-stage O\u003csub\u003e3\u003c/sub\u003e exposure[\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. Thus, recent preclinical research does indicate a path to gestational hypertension from early- or mid-gestational exposure to air pollution.\u003c/p\u003e \u003cp\u003eA missing component of the preclinical investigations into early gestational impacts of air pollution exposure on maternal cardiovascular health is a clear indicator of systemic hypertension, or blood pressure change, especially as develops in late pregnancy and resolves upon delivery (and removal of the offending placenta). In the present study, we therefore sought to assess O\u003csub\u003e3\u003c/sub\u003e exposure effects on blood pressure and related parameters using radiotelemetry for continuous measurement with minimal handling of the pregnant rats.\u003c/p\u003e"},{"header":"Materials and Methods","content":"\u003cp\u003e \u003cstrong\u003eAnimals\u003c/strong\u003e \u003cp\u003eFemale Sprague-Dawley rats were used in this study. Animals were housed in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-approved facility at the University of New Mexico Health Sciences Center and maintained at recommended temperature (20\u0026ndash;24\u0026deg;C), relative humidity (30\u0026ndash;60%), and on a 12-h light/dark cycle throughout the study. Animals were provided with standard chow and water ad libitum. All animal procedures were conducted humanely and approved by the Institutional Animal Care and Use Committee at the University of New Mexico.\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eRadiotelemetry Implantation\u003c/strong\u003e \u003cp\u003eRats were implanted with radiotelemetry devices for continuous acquisition of systemic arterial blood pressure, heart rate (HR), core body temperature (T\u003csub\u003eCO\u003c/sub\u003e), and physical activity (PA; HD-S10; DataSciences, Intl; St Paul, MN). Implantation was conducted as previously described. Briefly, under isoflurane anesthesia, the pressure catheter of the telemetry device was advanced through the insertion point of the femoral artery to a position within the abdominal aorta and secured with suture and tissue glue. The body of the telemeter was secured subcutaneously in the flank of the rat. Buprenorphine (0.03 mg/kg subcutaneous) was administered 30 min before the end of surgery and postoperatively as needed to provide analgesia. Rats recovered a minimum of 7 days prior to further experimentation.\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eMating\u003c/strong\u003e \u003cp\u003eOnce rats recovered from surgery, the fidelity of the blood pressure signal was confirmed (a minimum pulse pressure of 10 mmHg was required). Rats with viable blood pressure signals were then paired with male rats for up to 3 days, with confirmation of successful copulation as evidenced bu the presences of a seminal plug. Following successful plug check or after three days of pairing, female rats were removed and returned to normal housing conditions. Body weights were assessed at regular intervals following this pairing. A\u0026thinsp;\u0026gt;\u0026thinsp;5% increase in body weights at GD8.5 was consistent with pregnancy (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Pregnant rats were then exposed to O3 at mid gestation on GD10.5 (described below). Non-pregnant rats were considered for re-pairing, assuming the radiotelemetry signal remained strong. Three experiments were undertaken to obtain an N\u0026thinsp;=\u0026thinsp;7 pregnant rats per exposure group with radiotelemetry signals of sufficient quality. Following exposures, rats were followed to term and delivery.\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eO\u003csub\u003e3\u003c/sub\u003e Exposure\u003c/strong\u003e \u003cp\u003eDams were exposed to either filtered air (FA) or 1ppm O\u003csub\u003e3\u003c/sub\u003e for a single 4-h period via whole body inhalation. O\u003csub\u003e3\u003c/sub\u003e was generated using an OREC silent arc discharge generator (Osmonics, Phoenix, Arizona) as previously described [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e, \u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]. Water was available for animals during the exposure, but food was withheld. Exposures took place in standard shoebox cages without bedding (to prevent O\u003csub\u003e3\u003c/sub\u003e scavenging). O\u003csub\u003e3\u003c/sub\u003e concentrations were monitored in real-time using an indoor air quality monitor (GrayWolf Sensing Solutions, Shelton, CT) and adjustments to flow were conducted manually to ensure that concentrations were consistently between 0.9\u0026ndash;1.1 ppm.\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eData Analysis and Statistics\u003c/strong\u003e \u003cp\u003eData from all rats with sufficient signal quality was included (namely, pulse pressure\u0026thinsp;\u0026gt;\u0026thinsp;10 mmHg). Data for mean arterial blood pressure (MAP), HR, T\u003csub\u003eCO\u003c/sub\u003e, and PA were collected every 10 minutes, but averaged to daily values for statistical testing. FA and O\u003csub\u003e3\u003c/sub\u003e groups were compared by a 2-way repeated measures ANOVA to account for time and exposure, with Š\u0026iacute;d\u0026aacute;k's multiple comparisons test used to confirm O3 effects at specific time points (GraphPad Prism, v9.5.1). Data are presented as mean +/- SEM; p-values\u0026thinsp;\u0026lt;\u0026thinsp;0.05 were considered to be significant.\u003c/p\u003e \u003c/p\u003e"},{"header":"Results and Conclusions:","content":"\u003cp\u003e \u003cstrong\u003eAnimal Weights\u003c/strong\u003e \u003cp\u003eAnimals were weighed weekly to assess differences in weight gain and as a measure of overall health. Following pairing, female rats were weighed every 2\u0026ndash;4 days to assess likelihood of pregnancy (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Growth greater than 5% at GD8.5 allowed for a clear demarcation of pregnancy, at which dams were randomized for exposure conditions (FA or O\u003csub\u003e3\u003c/sub\u003e).\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eMean arterial blood pressure\u003c/strong\u003e \u003cp\u003eEvidence of a late-gestational increased in blood pressure was determined in O\u003csub\u003e3\u003c/sub\u003e-exposed rats (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e). Prior to exposures, the FA and O\u003csub\u003e3\u003c/sub\u003e groups, despite randomization, displayed an approximately 3.5 mmHg difference in mean arterial pressure, possibly real or due to calibration variance in the radiotelemetry devices. Regardless, following O\u003csub\u003e3\u003c/sub\u003e exposure at GD10.5, this difference became augmented by approximately 5.0 mmHg from approximately GD15.5 until delivery. To better ascertain the time frame of the increased mean arterial pressure, we normalized values to the period from GD3.5 to GD8.5, when paired mating was over but prior to exposures. With this approach, we revealed significant differences between FA and O\u003csub\u003e3\u003c/sub\u003e-exposed groups from GD15.5 until GD19.5. Notably, deliveries began between GD20.5 and GD22.5, at which point mean arterial pressure differences between exposure groups subsided.\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003ePA, HR, and T\u003csub\u003eCO\u003c/sub\u003e\u003c/strong\u003e \u003cp\u003eThe three additional parameters of PA, HR, and T\u003csub\u003eCO\u003c/sub\u003e all behaved differently compared to mean arterial pressure (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e). PA was generally not altered as a result of the O\u003csub\u003e3\u003c/sub\u003e exposure (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eA). Activity was elevated during pairing with male rats, and reduced for approximately 24h following the O\u003csub\u003e3\u003c/sub\u003e or sham exposure. Moreover, there was a steady reduction in PA throughout the course of pregnancy. Even with removing the first 4 days of data to eliminate effects of pairing with male rats, Time as a factor in the 2-way repeated measures ANOVA was significant (p\u0026thinsp;=\u0026thinsp;0.0013) while O\u003csub\u003e3\u003c/sub\u003e was not.\u003c/p\u003e \u003c/p\u003e \u003cp\u003eHR was acutely reduced by the O\u003csub\u003e3\u003c/sub\u003e exposure, on average 43 beats per minute lower than FA-exposed dams and fully resolving 48-72h post exposure (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eB). During the period of elevated mean arterial pressure (GD15.5-GD19.5), however, HR values were similar between exposure groups. This acute reduction in HR is consistent with numerous previous studies [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e]. Interestingly, HR increased in both groups peri-delivery, despite no increase in PA.\u003c/p\u003e \u003cp\u003eT\u003csub\u003eCO\u003c/sub\u003e was consistent between both groups prior to exposure, but displayed an interesting reduction late in pregnancy, significant on GD17.5 (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eC). This aligns well with the general period of elevated mean arterial blood pressure (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eB and C). Previous research has routinely described an acute hypothermia in mice and rats associated with O\u003csub\u003e3\u003c/sub\u003e exposure [\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e], but this delayed response appears unique to the pregnant rat. This acute phenomenon of hypothermia could be observed in the present study if data was viewed more closely (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eD). The drop in T\u003csub\u003eCO\u003c/sub\u003e was substantial (mean\u0026thinsp;=\u0026thinsp;33.2\u0026deg;C), but resolved within approximately 2h after the O\u003csub\u003e3\u003c/sub\u003e exposure ended.\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThe use of radiotelemetry in a Sprague Dawley rat model of pregnancy has been employed in several studies to evaluate changes in blood pressure, ostensibly to mimic gestational hypertension and/or preeclampsia. Infusion of the vasoconstrictor angiotensin II on gestational days 6, 9, 12, and 15 has been used to increase mean arterial blood pressure about 20\u0026thinsp;+\u0026thinsp;mmHg over controls, while maintaining the integrity of the pregnancy [\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. Severe hypertensive disorders of pregnancy, such as preeclampsia, have also been modeled utilizing intraperitoneal injection of the angiogenesis inhibitor Suramin to increase (17 mmHg) blood pressure [\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. To our knowledge, this is the first study utilizing radiotelemetry to continuously assess blood pressure of unrestrained pregnant rats in response to an inhaled toxicant such as ozone. Previous studies have examined blood pressure intermittently following ozone exposure in pregnant rats via tail cuff photoplethysmograpy and saw no blood pressure changes [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e], although slight differences in O\u003csub\u003e3\u003c/sub\u003e concentration as well as exposure timing complicate comparing the two studies.\u003c/p\u003e \u003cp\u003eO\u003csub\u003e3\u003c/sub\u003e exposure occurring during the initial phases of placentation (GD10.5) led to a delayed elevation of blood pressure in pregnant rats several days following the exposure. While the effect was mild (only a 5 mmHg increase), it was sustained until delivery, consistent with human gestational hypertension, i.e., elevated blood pressure of placental origin. The blood pressure changes were accompanied by a modest reduction in T\u003csub\u003eCO\u003c/sub\u003e, which may indicate a general morbidity associated with the late stage condition. Changes acutely caused by O\u003csub\u003e3\u003c/sub\u003e exposure, namely bradycardia and hypothermia, are typical of such exposures and rapidly resolved, as previously reported.[\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e] For reasons described below, the delayed pressor effect of O\u003csub\u003e3\u003c/sub\u003e in the present study is likely a unique pathology elicited by indirect effects on placental growth, setting the stage for a syndrome akin to gestational hypertension in late stages of pregnancy.\u003c/p\u003e \u003cp\u003eMothers are highly vulnerable to pathological cardiovascular and hemodynamic alterations during pregnancy, as the growing fetus and palcenta demand a substantive increase in cardiopulmonary output over the 9 months of gestation [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]. Under normal circumstances, maternal vascular resistance drops approximately 30% to accommodate increased demands on cardiac output from the growing fetus and the typical mild anemia that occurs. However, environmental factors may interfere with the normal vascular remodeling required for this reduction in systemic resistance, promoting hypertension and cardiomyopathy.\u003c/p\u003e \u003cp\u003ePlacental insufficiency is linked to maternal hypertension and preeclampsia. Preeclampsia occurs in roughly 3\u0026ndash;8% of pregnancies, reflecting a large population at risk, but numerous factors contribute to the etiology.[\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e] Incomplete angiogenesis during placentation can lead to a poorly perfused placenta, which responds by releasing proangiogenic and proinflammatory factors.[\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e] Maternal systemic blood pressure surges dramatically in preeclampsia, accompanied by proteinuria and elevations in several key circulating factors such as the soluble fms-like tyrosine kinase-1.[\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e] In addition to acute risk of seizure, death, or pre-term delivery resulting from a hypertensive crisis, long-term cardiomyopathic, hemodynamic, and cognitive deficits have been observed in preeclampsia patients post-delivery.[\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e, \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e] Preeclampsia incidence has a heterogeneous etiology and is influenced in part by genetics, immunology, nutritional status, and environmental exposures,[\u003cspan additionalcitationids=\"CR27 CR28 CR29\" citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e] but no single factor has a strong predictive role. The present study and other recent research may provide an integrated mechanistic understanding of how air pollution may adversely impact placental development, promoting preeclampsia and related maternal cardiovascular pathologies.\u003c/p\u003e \u003cp\u003eAs an important component of the mechanisms by which inhaled pollutants impact the systemic vasculature, we have identified that fragmented peptides arising from pulmonary peptidase activity in response to pollutants [\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e] can directly stimulate endothelial inflammatory responses in coronary, mesenteric, and cerebrovascular beds [\u003cspan additionalcitationids=\"CR33\" citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e], and there is little reason to assume that the placental vascular bed is not similarly vulnerable to the systemic effects of inhaled toxicants. As a specific example, a fragmented thrombospondin-1 peptide (corresponding to amino acids 402\u0026ndash;460) was highly upregulated in serum after pulmonary particulate matter exposure and was observed to reduce endothelial cell growth rates, most likely via interaction with the CD36 receptor.[\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e, \u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e] We postulate that such mechanisms may directly retard early placental growth especially of the vasculature. Later in gestation, the delayed placental growth may lead to release of pressors like sFlt-1 to direct greater blood flow to the placenta, elevating maternal vascular resistance and blood pressure (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eOne limitation to this study design is that blood pressure response to O\u003csub\u003e3\u003c/sub\u003e was not assessed in non-pregnant rats. Notably several studies have investigated this with limited indication that blood pressure or hemodynamics, in general, are impacted by O\u003csub\u003e3\u003c/sub\u003e. Numerous studies even point to a reduction in blood pressure in vulnerable or two-hit models designed to evince hypertension.[\u003cspan additionalcitationids=\"CR37 CR38 CR39\" citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e] Normally, O\u003csub\u003e3\u003c/sub\u003e is rapidly scavenged in the epithelial lining fluid of the lung and inflammatory/toxic effects are typically acute and resolved within 24\u0026ndash;72 hours, depending on the exposure concentration and extent of lung injury.[\u003cspan additionalcitationids=\"CR42\" citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e] The concentration used in the present study (1 ppm) typically resolves in 24h. Thus, the observation of significant mean arterial pressure changes, along with T\u003csub\u003eCO\u003c/sub\u003e changes, arising 5\u0026ndash;10 days after the exposure suggests there is a unique response in the setting of pregnancy that may promote the pathology of gestational hypertension. Early O\u003csub\u003e3\u003c/sub\u003e-induced deficits of placental growth and development might therefore set the stage for increased uterine artery resistance and ultimately higher blood pressure as demands of the fetus and placenta increase in late pregnancy.\u003c/p\u003e \u003cp\u003eA second limitation to this study is the rat model where the differences in gestational timing, litter size, and possibly placental physiology may limit translation to human gestation. However, the differences may also explain why the gestational hypertension seen in the present study was only a modest 5 mmHg, while human preeclampsia is characterized blood pressures exceeding 200 mmHg, \u003cem\u003ei.e\u003c/em\u003e., to life-threatening levels. The perfusion of the rodent uterus may become limited by the O\u003csub\u003e3\u003c/sub\u003e effect on uterine vascular resistance, but the rodent can reabsorb fetuses to balance this outcome and ensure a healthy supply of blood to all progeny, which humans are unable to do. Furthermore, the short duration of gestation (22 days) may simply not be sufficient to generate a profound hypertensive phenotype. Thus, we suspect the mild cardiovascular response in the rodent model implies that increasing the severity of O\u003csub\u003e3\u003c/sub\u003e exposure would lead to spontaneous abortion.\u003c/p\u003e \u003cp\u003eIn summary, pregnant rats exposed to O\u003csub\u003e3\u003c/sub\u003e at GD10.5 displayed a mild but significant increase in mean arterial blood pressure beginning 5 days after exposure and lasting until term. While this model has limitations and the scope of pathological assessment does not permit a stronger conclusion related to gestational hypertension or preeclampsia, the phenomenon of late-term elevated blood pressure that resolves after delivery is consistent with the debilitating and life-threatening human syndrome of preeclampsia. Integrated within the context of several recent research communications, this adds meaningfully to the understanding of how ambient air pollution may promote the risk of adverse pregnancy outcomes like gestational hypertension or spontaneous abortion. Much research is needed to better delineate the link between pulmonary exposures and placental effects as well as the relative contribution of summated pollutants, diet, genetics and lifestyle that may further contribute to risk of adverse outcomes.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003eThe authors declare they have no financial conflicts of interest with the content of this manuscript.\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eAll authors read and approved the manuscript.Major writing was conduct by RH, NLK, BB, and MC.Surgical procedures were conducted by CP, TW, and RHExposures were conducted by SLData collection and analysis was conducted by RH, DL, and MCStudy design and conceptualization was conducted by TW, RH, AO, MC, RA, and NLK\u003c/p\u003e\u003ch2\u003eAcknowledgements:\u003c/h2\u003e \u003cp\u003eThis reserch was funded by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (F31 HD107945), National Institute of Environmental Health Sciences (R01 ES014639) and the National Institute for General Medical Sciences (P20 GM130422).\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eBrook, R. 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M. \u003cem\u003eNovel Mechanisms of Ozone-Induced Pulmonary Inflammation and Resolution, and the Potential Protective Role of Scavenger Receptor BI\u003c/em\u003e. \u003cem\u003eResearch Report/Health Effects Institute\u003c/em\u003e, 2021(204): p. 1\u0026ndash;49.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"cardiovascular-toxicology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"cato","sideBox":"Learn more about [Cardiovascular Toxicology](http://link.springer.com/journal/12012)","snPcode":"12012","submissionUrl":"https://submission.nature.com/new-submission/12012/3","title":"Cardiovascular Toxicology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Ozone, Air Pollution, Pregnancy, Hypertension","lastPublishedDoi":"10.21203/rs.3.rs-3977101/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3977101/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe contribution of air pollution induced cardio-pulmonary damage on the development of hypertensive disorders of pregnancy and other adverse outcomes of pregnancy has gained increased attention as epidemiological data continues to highlight spatiotemporal pregnancy trends related to air pollution exposure. However clinical mechanistic data surrounding gestational complications remains sparse, necessitating the need for the use of animal models to study these types of complications of pregnancy. The current study seeks to examine the real-time effects of mid-gestational ozone exposure on maternal blood pressure and body temperature through the use of radiotelemetry in a rat model. The exposure resulted in acute depression of heart rate and core body temperature as compared to control animals. Ozone exposed animals also presented with a slight but significant increase in arterial blood pressure which was perpetuated until term. The data presented here illustrates the feasibility of murine models to assess cardiovascular complications caused by inhaled toxicants during the window of pregnancy.\u003c/p\u003e","manuscriptTitle":"Characterization of Mild Delayed Gestational Hypertension in Rats Following Ozone Exposure","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-02-23 12:17:22","doi":"10.21203/rs.3.rs-3977101/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-05-19T19:44:19+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-05-19T18:35:48+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"346cfc06-e695-410a-98c4-b80c6f93f053","date":"2024-04-15T11:33:36+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-03-08T13:23:15+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"1e2952d2-4e49-4256-b5dc-2326d09ed615","date":"2024-02-28T15:55:45+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-02-28T15:47:15+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-02-27T22:04:05+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-02-22T03:29:48+00:00","index":"","fulltext":""},{"type":"submitted","content":"Cardiovascular Toxicology","date":"2024-02-21T23:46:49+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"cardiovascular-toxicology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"cato","sideBox":"Learn more about [Cardiovascular Toxicology](http://link.springer.com/journal/12012)","snPcode":"12012","submissionUrl":"https://submission.nature.com/new-submission/12012/3","title":"Cardiovascular Toxicology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"1ee9b4a4-6012-4d50-b389-416b56904c2d","owner":[],"postedDate":"February 23rd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2024-06-27T12:41:50+00:00","versionOfRecord":[],"versionCreatedAt":"2024-02-23 12:17:22","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3977101","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3977101","identity":"rs-3977101","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}