The role of cydB gene in the biofilm formation by Campylobacter jejuni

The role of cydB gene in the biofilm formation by Campylobacter jejuni | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article The role of cydB gene in the biofilm formation by Campylobacter jejuni Jakub Korkus, Patrycja Sałata, Stuart A. Thompson, Emil Paluch, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4342718/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 04 Nov, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Campylobacter jejuni is a major cause of food- and water-borne bacterial infections in humans. A key factor helping bacteria to survive adverse environmental conditions is biofilm formation ability. Nonetheless, the molecular basis underlying biofilm formation by C. jejuni remains poorly understood. Around thirty genes involved in the regulation and dynamics of C. jejuni biofilm formation have been described so far. We applied random transposon mutagenesis to identify new biofilm-associated genes in C. jejuni strain 81-176. Of 1350 mutants, twenty-four had a decreased ability to produce biofilm compared to the wild-type strain. Some mutants contained insertions in genes previously reported to affect the biofilm formation process. The majority of identified genes encoded hypothetical proteins. In the library of EZ-Tn5 insertion mutants, we found the cydB gene associated with respiration that was not previously linked with biofilm formation in Campylobacter. To study the involvement of the cydB gene in biofilm formation, we constructed a non-marked deletion cydB mutant together with a complemented mutant. We found that the cydB deletion-mutant formed a weaker biofilm of loosely organized structure and lower volume than the parent strain. In the present study, we demonstrated the role of the cydB gene in biofilm formation by C. jejuni. Biological sciences/Microbiology/Bacteria Biological sciences/Microbiology/Biofilms C. jejuni biofilm transposon mutagenesis cydB Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 04 Nov, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Accepted 23 Oct, 2024 Reviews received at journal 30 Sep, 2024 Reviewers agreed at journal 20 Sep, 2024 Reviews received at journal 16 Sep, 2024 Reviewers agreed at journal 01 Sep, 2024 Reviewers invited by journal 21 Aug, 2024 Editor assigned by journal 21 Aug, 2024 Editor invited by journal 14 Aug, 2024 Submission checks completed at journal 13 Aug, 2024 First submitted to journal 29 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4342718","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":349631783,"identity":"6a7213cf-82d5-458c-aa30-712241bd3b67","order_by":0,"name":"Jakub Korkus","email":"","orcid":"","institution":"Wrocław University of Environmental and Life Sciences","correspondingAuthor":false,"prefix":"","firstName":"Jakub","middleName":"","lastName":"Korkus","suffix":""},{"id":349631784,"identity":"424a937c-162b-44c9-9679-792bf88d9866","order_by":1,"name":"Patrycja Sałata","email":"","orcid":"","institution":"Wrocław University of Environmental and Life Sciences","correspondingAuthor":false,"prefix":"","firstName":"Patrycja","middleName":"","lastName":"Sałata","suffix":""},{"id":349631785,"identity":"31fee100-e537-4bc5-8f1b-798457bea151","order_by":2,"name":"Stuart A. 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A key factor helping bacteria to survive adverse environmental conditions is biofilm formation ability. Nonetheless, the molecular basis underlying biofilm formation by C. jejuni remains poorly understood. Around thirty genes involved in the regulation and dynamics of C. jejuni biofilm formation have been described so far. We applied random transposon mutagenesis to identify new biofilm-associated genes in C. jejuni strain 81-176. Of 1350 mutants, twenty-four had a decreased ability to produce biofilm compared to the wild-type strain. Some mutants contained insertions in genes previously reported to affect the biofilm formation process. The majority of identified genes encoded hypothetical proteins. In the library of EZ-Tn5 insertion mutants, we found the cydB gene associated with respiration that was not previously linked with biofilm formation in Campylobacter. To study the involvement of the cydB gene in biofilm formation, we constructed a non-marked deletion cydB mutant together with a complemented mutant. We found that the cydB deletion-mutant formed a weaker biofilm of loosely organized structure and lower volume than the parent strain.\nIn the present study, we demonstrated the role of the cydB gene in biofilm formation by C. jejuni.","manuscriptTitle":"The role of cydB gene in the biofilm formation by Campylobacter jejuni","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-10 04:29:50","doi":"10.21203/rs.3.rs-4342718/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Accepted","date":"2024-10-23T05:39:15+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-30T04:54:37+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"82714684469942719251453040449504802248","date":"2024-09-20T06:01:27+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-17T02:43:04+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"73302354421373765426534124085518075342","date":"2024-09-01T22:23:02+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-08-21T13:55:47+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-08-21T13:42:03+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2024-08-14T14:30:23+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-08-13T10:09:33+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2024-04-29T11:42:21+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"c8d30cc3-1c50-4cc6-8ab1-7ea95f517313","owner":[],"postedDate":"September 10th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":37111082,"name":"Biological sciences/Microbiology/Bacteria"},{"id":37111083,"name":"Biological sciences/Microbiology/Biofilms"}],"tags":[],"updatedAt":"2024-11-11T16:03:32+00:00","versionOfRecord":{"articleIdentity":"rs-4342718","link":"https://doi.org/10.1038/s41598-024-77556-7","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2024-11-04 15:58:09","publishedOnDateReadable":"November 4th, 2024"},"versionCreatedAt":"2024-09-10 04:29:50","video":"","vorDoi":"10.1038/s41598-024-77556-7","vorDoiUrl":"https://doi.org/10.1038/s41598-024-77556-7","workflowStages":[]},"version":"v1","identity":"rs-4342718","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4342718","identity":"rs-4342718","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}