To Evaluate the Apoptosis Gene in Relation to Endometriosis

Objective To evaluate the molecular mechanism by measuring the gene expression of Survivin,Bcl-2 and Cox-2 in patients with endometriosis (EMS) and investigate their effects on the initiation and maintenance of endometriosis.Methods From January 2006 to November 2006,the ectopic endometrium and eutopic endometrium were obtained from 32 patients with endometriosis (EMS group) and from 32 normal endometrium(the control group).Informed consent was obtained from all patients. The mRNA expression of survivin,bcl-2 and cox-2 were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results Compared to normal group,the mRNA levels of survivin of ectopic endometrium and eutopic endometrium were significantly increased,the mRNA levels of bcl-2,cox 2 were significantly increased too.The total mRNA levels of survivin,bcl-2 and cox-2 were positively correlated with endometriosis,and the correlations of three were obvious.Conclusion Abnormal up-regulation of Survivin,Bcl-2 and Cox-2 in the endometriosis patients might be one of molecular biologic mechanisms for ecotopic implantation and development of endometrium.Furthermore, three genes were highly associated to the endometriosis progress.