Establishment and dynamic histopathology observation of rats model with endometritis

【Objective】 The research was to establish animal model of endometritis in rats to study the pathogenesis of the cow endometriosis,and to provide an in vitro model for prevention and treatment of the disease.【Method】 30 clean female rats were randomly divided into inoculated group with 22 and control group with 8.The mixed pathogenic bacteria of Escherichia coli,Staphylococcus aureus and Streptococcus agalactiae(bacteria liquid volume proportion was 2∶1∶1) were inoculated into uterine cavity using surgery method,while the control group was not inoculated.The macroscopic anatomical lesions,histopathological change and uterine index were detected on the 3rd,6th,9th,12th day post inoculation(d.p.i.),and pathogenic bacteria were isolated from uterine elution at different time points also.【Result】 The uterine index of infected rats increased more significantly than that of the control on the 3rd to 12th days post inoculation(P0.01).Uterus of infected rats showed acute catarrhal inflammation(3 and 6 d.p.i.),suppurative inflammation(9 d.p.i.) and chronic proliferative inflammation(12 d.p.i.) with the progress of the infection.The damage of uterus including uterine epithelial cells shedding,hyperaemia,haemorrhage,and inflammation cells infiltrates was observed at early stage of infection,and muscularis thickening was shown later on.【Conclusion】 Rat model of endometritis was established by using surgery method,and the appropriate time for development of rat model of endometritis was on the 3rd day post inoculation.