Additional file 5: of miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells

Figure S3. miR-205-5p knockdown promoted migration and invasion but suppressed apoptosis of EN211 and EN307 in vitro, related to Fig. 2. a. miR-205-5p levels in EN211 and EN307 stably transfected with anti-205-5p (anti-205) or negative control (anti-NC) lentivectors were detected by qRT-PCR. b. Representative micrographs of wound healing assay in miR-205-5p-knockdown EN211 and EN307 compared with NC were shown. Images were acquired at 0 (white dotted line) and 48 h (black dotted line). Average migration rate per field was calculated. Scale bar, 20 Îźm. c. Representative micrographs of Transwell invasion assay in miR-205-5p- knockdown EN211 and EN307 compared with NC were shown. Average invasive cells per field were calculated. Scale bar, 50 Îźm. d. Representative micrographs of apoptosis assay in miR-205-5p-knockdown EN211 and EN307 compared with NC were shown. Average apoptosis rate per time was analysed. anti-205, anti-miR-205-5p. Error bars represent the mean Âą SD of three independent experiments. **, P<0.01; ***, P<0.001. (TIF 4383 kb)