MOESM9 of An assessment of the multifactorial profile of steroid-metabolizing enzymes and steroid receptors in the eutopic endometrium during moderate to severe ovarian endometriosis

Additional file 9: Figure S2. Representative images of Western blots showing the effect of the menstrual cycle phase on the levels of the SF-1, StAR, aromatase, 17β-HSD1, 17β-HSD2, ERα, Erβ, PRA and PRB proteins in endometrial samples from the control, proliferative (C,P; group 1C); control, secretory (C,S; group 1D); OE, proliferative (OE,P; group 2C); and OE, secretory (OE,S; group 2D) groups. Tissue lysates of samples from these groups (25 μg of protein, concentrations were determined by using the Bradford assay) were subjected to electrophoretic separation followed by immunoblot analysis. The relative optical densities were measured by performing an integrated image analysis and normalizing the values to the μg of total protein.