Changes in connexin 43 protein expression in human endometrial carcinoma

Scott R. Schlemmer, S R Schlemmer, D B Novotny, Debra B. Novotny, David G. Kaufman, D G Kaufman
Experimental and molecular pathology · 1999 · vol. 67(3) , pp. 150–163 · doi:10.1006/exmp.1999.2286 · PMID:10600398 · W2074543724
Other OA: closed CC0
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-08-08 ⓘ

Connexin 43 expression was inversely correlated with endometrial carcinoma grade in cell lines and human tissue samples, suggesting its potential as a marker of tumor progression.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

The expression of connexin 43 was studied using immunohistochemical and Western blot analyses on cell lines of endometrial epithelial origin. Connexin proteins were examined because decreases in their expression and function have been correlated with carcinogenesis. The cell lines were chosen to represent increasing grades of endometrial cancer progression starting from FEEC (fetal endometrial epithelial cells; transformed with SV40 large T antigen) to HEC-1A (stage 1A endometrial carcinoma) to RL-95-2 (grade 2 endometrial carcinoma). Parallel studies using connexin 43 polyclonal antibodies for both Western blots and immunofluorescence showed that the levels of connexin 43 expression were normal endometrial stromal cells = FEEC > HEC-1A > RL-95-2. Consequently, we applied the immunofluorescence assay to analyze paraffin-embedded uterine sections from hysterectomy specimens of patients with normal endometrium and from patients diagnosed with grade 1, 2, and 3 endometrial cancer. Using five different cases from each category, we found an inverse correlation between connexin 43 expression and tumor grade. Our data indicate that connexin 43 expression may be useful as an adjunctive marker of progression for endometrial carcinoma.

My notes (saved in your browser only)

MeSH descriptors

Connexin 43 Endometrial Neoplasms Adenocarcinoma Adenocarcinoma Adenocarcinoma Blotting, Western Cell Line Connexin 43 Endometrial Neoplasms Endometrial Neoplasms Endometrium Endometrium Epithelial Cells Epithelial Cells Female Humans Microscopy, Fluorescence

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.

References (18)

Source provenance

openalex
last seen: 2026-06-04T00:00:01.174412+00:00
pubmed
last seen: 2026-10-08T20:59:14.592962+00:00
unpaywall
last seen: 2026-11-04T06:24:41.714486+00:00
License: CC0 · commercial use OK