High intensity focused ultrasound ablation for human adenomyosis in vitro

Objective To investigate the efficacy and clinical feasibility of high intensity focused ultrasound(HIFU)ablation for the human adenomyosis in vitro.Methods Seven human adenomyosis specimens were ablated with HIFU,and maximal ranges of hyperecho in ablated masses were detected with sonography.Then the specimens were stained in TTC(2,3,5-Triphenyltetrazolium chloride)solution,and maximal ranges were measured where TTC staining was negative.The results obtained by two methods were compared.At last,the tissues and cells in and around the ablated masses were observed under light microscope.Results The regions of adenomyosis in the specimens were hypoechoic heterogeneously with sonography before ablation,and the hyperechoic regions were detected in all ablated areas.The ablated regions in the specimens became gray-yellow and hardened,while the regions out of the ablation had no obvious change with eyes.The TTC staining was negative in the ablated lesion where histological examination revealed coagulation necrosis and the stained regions had no obvious change in histology.There was no difference between the two average maximal ablated ranges(P0.05).Conclusion HIFU can ablate human adenomyosis in vitro effectively and do not hurt the tissues around of the ablation.