Identification of SBP Gene Family and Analysis of Expression Patterns under salt stress in Quinoa 1

This study identified the SQUAMOSA Promoter Binding Proteins transcription factor SBP from the whole genome of Quinoa by analyzing the phylogenetic relationship, gene structure, chromosome location and gene replication, upstream cis-regulatory element, tissue expression, and construction of the SBP protein interaction network, as well as analyzing the characteristics of the SBP tissue expression pattern under salt stress. It serves as the foundation for the study and application of Quinoa SBP. The results showed that 23 genes of the CqSBP family were identified by bioinformatics analysis, and their amino acid physical and chemical properties showed diversity. The family proteins were hydrophilic, and the subcellular localization showed that 23 CqSBPs were located in the nucleus. The SBP family genes are unevenly distributed on the 12 chromosomes of Quinoa; the upstream cis-acting element analysis shows that there are 49 elements with plant hormones, stress, light response, and tissue-specific expression, and all CqSBPs contain one or more TATA box elements. Protein interaction network analysis shows that all CqSBP proteins appear in the known interaction network of Arabidopsis; the expression of different SBP genes in different organs and periods of Quinoa is different, and the expression of SBP genes has certain tissue specificity. The expression of CqSBP showed many changes under salt stress.