Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants

This study aimed to establish a simple and efficient in vivo multiplication protocol by leaf cutting to satisfy the supply of young succulent ornamentals Pachyveria pachytoides and Sedum morganianum . The regenerability of leaves injected with 6-benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) in vivo were tested with common leaf cutting as control. Results showed a 100% shoot induction frequency using hormone-injeceted methods for the two species. The number of shoots per leaf of 4.0 or 6.0 mg l −1 BAP and 0.1 mg l −1 NAA injected in vivo (5.08-5.14 in P. pachytoides , and 6.22-6.74 for S. morganianum) were significantly greater than that of the other treatments. Since the h ormone-injected leaf cutting needs no aseptic operation which is necessary for in vitro multiplication, it is simple for the commercial production of the two species. The new in vivo propagation method would be of great interest for growers and breeders of succulent plants.