Whole-Genome Sequencing and Genomic Mining of a Multidrug-Resistant Lactose-Fermenting Enterobacteriaceae Strain Isolated from a Nosocomial Infection

Whole-Genome Sequencing and Genomic Mining of a Multidrug-Resistant Lactose-Fermenting Enterobacteriaceae Strain Isolated from a Nosocomial Infection | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 28 June 2025 V1 Latest version Share on Whole-Genome Sequencing and Genomic Mining of a Multidrug-Resistant Lactose-Fermenting Enterobacteriaceae Strain Isolated from a Nosocomial Infection Authors : Noor Radhwan Abdulkadhim ALBUNSRALLA 0009-0003-2861-8400 [email protected] , Abeer Babiker Idris , Pınar SAĞIROĞLU , Mustafa Altay ATALAY , and Abdurrahman Ayvaz Authors Info & Affiliations https://doi.org/10.22541/au.175109989.95872223/v1 222 views 110 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The routine phenotypic methods originally identified the NA22 isolates as Klebsiella pneumoniae . Nevertheless, WGS and NCBI annotation have subsequently confirmed that the microorganism is an Escherichia coli . The isolate had been recovered from the bloodstream of a patient and was resistant to many of the most important classes of antibiotics. A total of eight contigs >1,000 bp were generated through sequencing on the Oxford Nanopore platform. Three plasmids, NA22_1, NA22_2, and NA22_3, were identified using PlasmidFinder. Among them, NA22_3 harbored significant resistance genes, including blaNDM-4, blaCTX-M-15, and aac(6’)-Ib-cr, along with the tra operon genes that may facilitate plasmid transfer. Functional annotation was performed against the PATRIC, CARD, and VFDB databases, and 76 resistance and over 200 virulence, as well as over 100 metabolism and transport involved subsystems, were found. The antimicrobial susceptibility tests revealed resistance to β-lactams (including carbapenems), fluoroquinolones, aminoglycosides, and sulfonamides, which were consistent with the genome profile. The complete genome sequence is included in BioProject ID: PRJNA1270365. These results highlight the importance of routine genomic surveillance of multidrug-resistant strains within hospitals. While bioinformatics analyses highlighted the critical importance of routine monitoring of multidrug-resistant strains in the hospital environment, these findings particularly demonstrated the necessity of careful surveillance and personalized treatment strategies to combat the spread of these identified bacteria in clinical settings. Supplementary Material File (figures.docx) Download 1.47 MB File (manuscript.docx) Download 59.00 KB File (tables.docx) Download 79.64 KB Information & Authors Information Version history V1 Version 1 28 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords aac(6’)-ib-cr antimicrobial resistance blandm-4 genome sequencing multidrug resistance Authors Affiliations Noor Radhwan Abdulkadhim ALBUNSRALLA 0009-0003-2861-8400 [email protected] Erciyes Universitesi Fen Bilimleri Enstitusu View all articles by this author Abeer Babiker Idris Erciyes Universitesi Fen Bilimleri Enstitusu View all articles by this author Pınar SAĞIROĞLU Erciyes Universitesi View all articles by this author Mustafa Altay ATALAY Erciyes Universitesi View all articles by this author Abdurrahman Ayvaz Erciyes Universitesi View all articles by this author Metrics & Citations Metrics Article Usage 222 views 110 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Noor Radhwan Abdulkadhim ALBUNSRALLA, Abeer Babiker Idris, Pınar SAĞIROĞLU, et al. Whole-Genome Sequencing and Genomic Mining of a Multidrug-Resistant Lactose-Fermenting Enterobacteriaceae Strain Isolated from a Nosocomial Infection. Authorea . 28 June 2025. 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