CeBiNES-02, a herbal Mixture, enhances skin barrier function and alleviates atopic dermatitis symptoms in mice

CeBiNES-02, a herbal Mixture, enhances skin barrier function and alleviates atopic dermatitis symptoms in mice | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article CeBiNES-02, a herbal Mixture, enhances skin barrier function and alleviates atopic dermatitis symptoms in mice Soyeon Kim, Jimi Lee, Jinhu Peng, Yanfeng Ren, Jung-Hoon Kim, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7930444/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 7 You are reading this latest preprint version Abstract The constituents of CeBiNES-02 (C02) are categorized as 'heat-clearing' herbs within the framework of Northeast Asian traditional medicine, or have historically been employed in the management of dermatological conditions. This study aimed to evaluate the anti-inflammatory properties and skin barrier-protective effects of C02 in a mouse model of calcipotriol (MC903) induced atopic dermatitis (AD). C02 was applied topically to the backs of 8-week-old male Balb/c mice at three different doses. Skin lesion severity, skin water content, water-holding capacity (WHC), histopathological abnormalities, and inflammatory cytokine levels were analyzed after 6 days of C02 application. The results demonstrated that C02 effectively alleviated the severity of skin lesions and erythema, increased skin water content and WHC, and ameliorated histopathological abnormalities viz. spongiotic changes, basal layer disruption, and immune cell infiltration in inflamed tissues. Furthermore, C02 tended to restore filaggrin expression and suppressed the production of inflammatory cytokines, namely TNF (tumor necrosis factor)-α, IFN (interferon)-γ, and IL (interleukin)-4 and IL-6. In addition, C02 reduced TSLP (thymic stromal lymphopoietin) expression and inhibited the phosphorylation of ERK (extracellular signal-regulated kinase). In Conclusion, these findings suggest that C02 has therapeutic potential as an anti-inflammatory and skin barrier protective agent in AD. Health sciences/Diseases Biological sciences/Drug discovery Biological sciences/Immunology Health sciences/Medical research Herbal mixture inflammation skin barrier function atopic dermatitis Full Text Additional Declarations No competing interests reported. Supplementary Files Suppdataupdate.zip Originalblotgels.pdf Cite Share Download PDF Status: Under Review Version 1 posted Reviews received at journal 07 Apr, 2026 Reviewers agreed at journal 02 Apr, 2026 Reviewers invited by journal 02 Apr, 2026 Editor assigned by journal 18 Feb, 2026 Editor invited by journal 06 Nov, 2025 Submission checks completed at journal 31 Oct, 2025 First submitted to journal 31 Oct, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7930444","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":618039523,"identity":"d3254d23-2c2e-4dce-875d-f813f2c48338","order_by":0,"name":"Soyeon Kim","email":"","orcid":"","institution":"Pusan National University","correspondingAuthor":false,"prefix":"","firstName":"Soyeon","middleName":"","lastName":"Kim","suffix":""},{"id":618039527,"identity":"80c848fc-cb2c-4e56-bf1d-138199d9510c","order_by":1,"name":"Jimi Lee","email":"","orcid":"","institution":"Pusan National 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This study aimed to evaluate the anti-inflammatory properties and skin barrier-protective effects of C02 in a mouse model of calcipotriol (MC903) induced atopic dermatitis (AD). C02 was applied topically to the backs of 8-week-old male Balb/c mice at three different doses. Skin lesion severity, skin water content, water-holding capacity (WHC), histopathological abnormalities, and inflammatory cytokine levels were analyzed after 6 days of C02 application. The results demonstrated that C02 effectively alleviated the severity of skin lesions and erythema, increased skin water content and WHC, and ameliorated histopathological abnormalities viz. spongiotic changes, basal layer disruption, and immune cell infiltration in inflamed tissues. Furthermore, C02 tended to restore filaggrin expression and suppressed the production of inflammatory cytokines, namely TNF (tumor necrosis factor)-α, IFN (interferon)-γ, and IL (interleukin)-4 and IL-6. 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