Cell Sorting On The Basis Of Viability And Size

Cell Sorting On The Basis Of Viability And Size | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Cell Sorting On The Basis Of Viability And Size Swapnil Namekar, Deepak Bankar, Vishal Mehtre, Yogesh Chendake This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7218467/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Stem cells are known to be the life starting cells. They have detail information of human life, organs, their growth, recovery and healing. They possess the ability of re-growth and regeneration of body part. Thus they can be useful in organ replacement and treatment of body parts damaged due to internal or external causes. They have applicability in human life saving and improvement treatments. Stem cell therapy is emerging as main treatment process in many of these cases where there is organ damage or major accidents causing threat to human life. Additionally, many applications are emerging for treatment of various diseases. They include all kinds of diseases from Alzheimer’s, Type-1 Diabetes, Heart disease, Strokes to dental treatment. Though stem cell therapy has multiple applications, it requires segregation of cells for effective treatment. There is need of cell sorting, which is highly important for effective treatments. A care must be taken during the storage, sorting and handling of these cells for maintaining cell viability as it can affect cell applicability. The cell sorting on the basis of viability and size could be done using Di-electrophoresis and Hydrodynamics. Hence a methos for sorting the cells on the basis of viability by dual frequency di-electrophoresis (DFDEP) technique and on the basis of size by hydrodynamic technique is used here. DFDEP is based on the concept of an effective Clausius–Mossotti factor, CMeff, for a particle that is exposed to the electric fields of different frequencies by using pairs of electrodes. The cell sorting can be done by combining DEP and hydrodynamic techniques. The separation of particles with very similar CM and trapping multiple groups of cells can be done simultaneously and later the size based sorting can be achieved with high resolution. The demand for effective cellular separation continue to escalate in response to growing applicability and importance of cellular therapies. In this context, the present study explores the potential of DFDEP towards separation of cells based on viability, while polysulfone (PSF) porous membranes can be utilized for size based separations. These membranes demonstrate superior performance in the segregations of live-dead cells of required size in presence of dual di-electrophoresis as seen from charged particles separation which can mimic cellular response. This is highly important in highlighting their potential for application in health care sector. However, further research is warranted to optimize synthesis parameters, enhance membrane performance, and address practical implementation challenges for cellular therapies through invitro and invivo analysis. Overall, this research opens an arena for combining membrane technology for cellular manipulation, offering insights into the development of effective and biocompatible solutions for addressing cellular sorting without affecting the cell physiological properties. Biological sciences/Biological techniques Biological sciences/Biotechnology Biological sciences/Cell biology Physical sciences/Engineering Biological sciences/Stem cells Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7218467","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":503372776,"identity":"9484ddb5-1e8a-4175-9279-685e2b991b6e","order_by":0,"name":"Swapnil Namekar","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA9klEQVRIiWNgGAWjYPCCBAYG9h4EVwKMCGrhOUOyFokcFC24gXz72YOfblSkyZvPfHtMmqfGRs6cgfngbR4GizxcWgzO5CVL55zJMZxzOy9NmudYmrFlA1uyNQ+DRDFOLQw5BtK5bRWMM6RzjI15Gw4nbjjAYyYN1JLYgMth/W+Mf+f+q7CfIXkGrKV+wwH+b3i1MNzIMZPObchJnCHBY/gYqCXB4AAPG14tBjfemFnnHEtLnsGTl/hwzrE0w53NbMaWcwzwOSzH+HZOTbLtDPazBw68qbGRN2dvfnjjTUUdbodh2ssMJolWT6riUTAKRsEoGBEAABuYT9ko/6lnAAAAAElFTkSuQmCC","orcid":"","institution":"Bharati Vidyapeeth (Deemed to be University","correspondingAuthor":true,"prefix":"","firstName":"Swapnil","middleName":"","lastName":"Namekar","suffix":""},{"id":503372777,"identity":"470ca5f4-92e0-440f-91e7-95a4c417374f","order_by":1,"name":"Deepak Bankar","email":"","orcid":"","institution":"Bharati Vidyapeeth (Deemed to be University","correspondingAuthor":false,"prefix":"","firstName":"Deepak","middleName":"","lastName":"Bankar","suffix":""},{"id":503372778,"identity":"cf9584a4-1e6d-4817-a2b4-44b5d8800dfe","order_by":2,"name":"Vishal Mehtre","email":"","orcid":"","institution":"Bharati Vidyapeeth (Deemed to be University","correspondingAuthor":false,"prefix":"","firstName":"Vishal","middleName":"","lastName":"Mehtre","suffix":""},{"id":503372779,"identity":"da0b9b1c-c449-4563-b454-142c4da9be7a","order_by":3,"name":"Yogesh Chendake","email":"","orcid":"","institution":"Bharati Vidyapeeth (Deemed to be University","correspondingAuthor":false,"prefix":"","firstName":"Yogesh","middleName":"","lastName":"Chendake","suffix":""}],"badges":[],"createdAt":"2025-07-26 04:53:14","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7218467/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7218467/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":89892104,"identity":"979c3b22-0831-4ec6-afa0-91e1310899c4","added_by":"auto","created_at":"2025-08-26 07:47:55","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":654256,"visible":true,"origin":"","legend":"","description":"","filename":"Cellsorterpaper05082025SAN.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7218467/v1_covered_c18e05f0-9c3d-4760-a848-570f49028498.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Cell Sorting On The Basis Of Viability And Size","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-7218467/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7218467/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eStem cells are known to be the life starting cells. They have detail information of human life, organs, their growth, recovery and healing. They possess the ability of re-growth and regeneration of body part. Thus they can be useful in organ replacement and treatment of body parts damaged due to internal or external causes. They have applicability in human life saving and improvement treatments. Stem cell therapy is emerging as main treatment process in many of these cases where there is organ damage or major accidents causing threat to human life. Additionally, many applications are emerging for treatment of various diseases. They include all kinds of diseases from Alzheimer\u0026rsquo;s, Type-1 Diabetes, Heart disease, Strokes to dental treatment. Though stem cell therapy has multiple applications, it requires segregation of cells for effective treatment. There is need of cell sorting, which is highly important for effective treatments. A care must be taken during the storage, sorting and handling of these cells for maintaining cell viability as it can affect cell applicability. The cell sorting on the basis of viability and size could be done using Di-electrophoresis and Hydrodynamics. Hence a methos for sorting the cells on the basis of viability by dual frequency di-electrophoresis (DFDEP) technique and on the basis of size by hydrodynamic technique is used here. DFDEP is based on the concept of an effective Clausius\u0026ndash;Mossotti factor, CMeff, for a particle that is exposed to the electric fields of different frequencies by using pairs of electrodes. The cell sorting can be done by combining DEP and hydrodynamic techniques. The separation of particles with very similar CM and trapping multiple groups of cells can be done simultaneously and later the size based sorting can be achieved with high resolution.\u003c/p\u003e\u003cp\u003eThe demand for effective cellular separation continue to escalate in response to growing applicability and importance of cellular therapies. In this context, the present study explores the potential of DFDEP towards separation of cells based on viability, while polysulfone (PSF) porous membranes can be utilized for size based separations. These membranes demonstrate superior performance in the segregations of live-dead cells of required size in presence of dual di-electrophoresis as seen from charged particles separation which can mimic cellular response. This is highly important in highlighting their potential for application in health care sector.\u003c/p\u003e\u003cp\u003eHowever, further research is warranted to optimize synthesis parameters, enhance membrane performance, and address practical implementation challenges for cellular therapies through invitro and invivo analysis. Overall, this research opens an arena for combining membrane technology for cellular manipulation, offering insights into the development of effective and biocompatible solutions for addressing cellular sorting without affecting the cell physiological properties.\u003c/p\u003e","manuscriptTitle":"Cell Sorting On The Basis Of Viability And Size","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-08-22 12:23:16","doi":"10.21203/rs.3.rs-7218467/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"3e446e64-23f3-471c-8a61-d54392963871","owner":[],"postedDate":"August 22nd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":53474879,"name":"Biological sciences/Biological techniques"},{"id":53474880,"name":"Biological sciences/Biotechnology"},{"id":53474881,"name":"Biological sciences/Cell biology"},{"id":53474882,"name":"Physical sciences/Engineering"},{"id":53474883,"name":"Biological sciences/Stem cells"}],"tags":[],"updatedAt":"2025-08-26T07:39:38+00:00","versionOfRecord":[],"versionCreatedAt":"2025-08-22 12:23:16","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7218467","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7218467","identity":"rs-7218467","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}