AI-Assisted Identification of Primary and Secondary Metabolomic Markers for Post-Operative Delirium

AI-Assisted Identification of Primary and Secondary Metabolomic Markers for Post-Operative Delirium | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article AI-Assisted Identification of Primary and Secondary Metabolomic Markers for Post-Operative Delirium Vladimir Lomivorotov, Vladimir Ivanisenko, Aelita-Luiza Makarova, and 14 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3848065/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Despite considerable investigative efforts, the molecular mechanisms of postoperative delirium (POD) remain unresolved. The present investigation employs innovative methodologies for identifying potential primary and secondary metabolic markers of POD by analyzing serum metabolomic profiles utilizing the genetic algorithm and artificial neural networks. The primary metabolomic markers constitute a combination of metabolites that optimally distinguish between POD and non-POD groups of patients. Our analysis revealed L-lactic acid, inositol, and methylcysteine as the most salient primary markers, upon which the prediction accuracy of POD manifestation achieved AUC = 99%. The secondary metabolomic markers represent metabolites that exhibit perturbed correlational patterns within the POD group. We identified 54 metabolites as the secondary markers of POD, incorporating neurotransmitters such as gamma-aminobutyric acid (GABA), serotonin. These findings imply a systemic disruption in metabolic processes in patients with POD. The deployment of gene network reconstruction techniques facilitated the postulation of hypotheses describing the role of established genomic POD markers in the molecular-genetic mechanisms of metabolic pathways dysregulation, involving the identified primary and secondary metabolomic markers. This study not only expands the understanding of POD pathogenesis but also introduces a novel technology for bioinformatic analysis of metabolomic data which could aid in uncovering potential primary and secondary markers in diverse research domains. Biological sciences/Computational biology and bioinformatics/Gene regulatory networks Health sciences/Biomarkers Full Text Additional Declarations There is NO Competing Interest. Supplementary Files DeliriumSupplementaryTables.xlsx Dataset 1 Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3848065","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":267711378,"identity":"f954dabd-64a9-4d83-ab03-c21ad49db7ca","order_by":0,"name":"Vladimir Lomivorotov","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA40lEQVRIiWNgGAWjYFAC5gYGBgMYpwIkABLBCxhhWpiB+AyIZiRGCwNUC2Mbigh2YHCAsfHRjYLDcubt/Qcf886rjeZvB2r5UbENn5Zm4xyDw8YyZw4zG/NuO5474zBjA2PPmds4tUg2MLZJ5xikJc6QSGaTnLntWG4DUAszYxvRWuYcy51PSAs/A1iLDViLxMeGmtwNBLUwg/1iYyzBc9jY4MOxA7kbgVoO4vMLG3vzwcc5fyTkJNgbHz5IqKnLnXf+8MEHPypwawFHIBI4DCYP4FaPCepIUTwKRsEoGAUjBAAAYkVTXjrgCgUAAAAASUVORK5CYII=","orcid":"","institution":"Penn State Hershey Medical Center, Penn State College of Medicine","correspondingAuthor":true,"prefix":"","firstName":"Vladimir","middleName":"","lastName":"Lomivorotov","suffix":""},{"id":267711379,"identity":"3090d35e-2358-4cca-adaa-fe652b214362","order_by":1,"name":"Vladimir Ivanisenko","email":"","orcid":"","institution":"Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk State University, Kurchatov Genomic Center of ICG SB RAS, Novosibirsk, Russia","correspondingAuthor":false,"prefix":"","firstName":"Vladimir","middleName":"","lastName":"Ivanisenko","suffix":""},{"id":267711380,"identity":"52a70923-6d37-4ff6-9b2b-d716464eecca","order_by":2,"name":"Aelita-Luiza Makarova","email":"","orcid":"","institution":"Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia","correspondingAuthor":false,"prefix":"","firstName":"Aelita-Luiza","middleName":"","lastName":"Makarova","suffix":""},{"id":267711381,"identity":"3b1ada1d-2d65-405d-ad36-6c2ec2e9394a","order_by":3,"name":"Artem Rogachev","email":"","orcid":"https://orcid.org/0000-0002-3338-8529","institution":"Novosibirsk State University, N. 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