Optimization of Super Oxidized Water Redox Properties by DOE for Targeted Disinfection Applications

Super oxidized water is a disinfectant agent generated by electrolysis. Its effectiveness de-pends mainly on the oxidation-reduction potential and pH. In the present study, a 22 fac-torial Design of Experiments was used in order to evaluate the influence of the applied potential and the NaCl concentration on the ORP and pH of super oxidized water, with the aim of generating solutions with specific redox values for different disinfection appli-cations. The models obtained showed a high predictive capacity (R2 > 0.99), identifying NaCl concentration as the factor with the greatest effect on the oxidation-reduction poten-tial and pH. The optimized conditions presented experimental errors of less than 1.5%, thus confirming the validity of the model. The solutions showed high physicochemical stability during 24 weeks of storage. Microbiological evaluation showed antimicrobial ac-tivity against Escherichia coli, Staphylococcus aureus, Methicillin-Resistant Staphylococcus au-reus, and Candida albicans, with its responses being dependent on the ORP level and the microorganism evaluated. The results demonstrate that the use of DOE allows for the ad-justment of redox profile of super oxidized water in a controlled manner for specific ap-plications, simultaneously optimizing antimicrobial efficacy, which positions super oxi-dized water as a flexible and scalable technology for disinfection in industrial and clinical contexts.