Microbial Desalination Using Advenella faeciporci ZF1: A Novel Iron- Reducing Bacterium Isolated from Oil Refinery Sludge

preprint OA: closed
Full text JSON View at publisher

Abstract

Abstract Advenella faeciporci strain ZF1, a novel isolated iron-reducing bacterium from Esfahan Oil Refining sludge, exhibited remarkable tolerance to FeCl₃ (up to 20 g/L) and electrochemical activity under aerobic and anaerobic conditions. ZF1 was phylogenetically identified and characterized via FTIR, polyhydroxybutyrate (PHB) accumulation, and magnetotactic behavior. Integrated into a microbial desalination cell (MDC), ZF1 achieved sodium, calcium, and chloride removal efficiencies of 70.6%, 78.6%, and 27.5% in refinery brine over 15 days. Under 9 g/L NaCl salinity, the system generated a maximum voltage of 410 ± 15 mV within the first 48 h. The corresponding peak power density reached 92 ± 5 mW/m² at an external resistance of 100 Ω, and the calculated current density was 1.80 ± 0.1 A/m². These findings highlight A. faeciporci ZF1 as a promising exoelectrogen for sustainable water desalination and metal-rich wastewater bioremediation.
Full text 21,821 characters · extracted from preprint-html · click to expand
Microbial Desalination Using Advenella faeciporci ZF1: A Novel Iron- Reducing Bacterium Isolated from Oil Refinery Sludge | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Microbial Desalination Using Advenella faeciporci ZF1: A Novel Iron- Reducing Bacterium Isolated from Oil Refinery Sludge Zarrindokht Emami-Karvani, Giti Emtiazi, Iraj Nahvi, Alimohammad Ahadi, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7905108/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 14 You are reading this latest preprint version Abstract Advenella faeciporci strain ZF1, a novel isolated iron-reducing bacterium from Esfahan Oil Refining sludge, exhibited remarkable tolerance to FeCl₃ (up to 20 g/L) and electrochemical activity under aerobic and anaerobic conditions. ZF1 was phylogenetically identified and characterized via FTIR, polyhydroxybutyrate (PHB) accumulation, and magnetotactic behavior. Integrated into a microbial desalination cell (MDC), ZF1 achieved sodium, calcium, and chloride removal efficiencies of 70.6%, 78.6%, and 27.5% in refinery brine over 15 days. Under 9 g/L NaCl salinity, the system generated a maximum voltage of 410 ± 15 mV within the first 48 h. The corresponding peak power density reached 92 ± 5 mW/m² at an external resistance of 100 Ω, and the calculated current density was 1.80 ± 0.1 A/m². These findings highlight A. faeciporci ZF1 as a promising exoelectrogen for sustainable water desalination and metal-rich wastewater bioremediation. Biological sciences/Biotechnology Earth and environmental sciences/Environmental sciences Biological sciences/Microbiology Microbial Desalination Cells (MDCs) Iron-Reducing Bacteria (IRB) Bioelectricity generation Electroactive Bacteria in Desalination Microbial fuel cells (MFCs) Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 10 Dec, 2025 Reviews received at journal 01 Dec, 2025 Reviews received at journal 30 Nov, 2025 Reviewers agreed at journal 28 Nov, 2025 Reviews received at journal 26 Nov, 2025 Reviewers agreed at journal 25 Nov, 2025 Reviewers agreed at journal 24 Nov, 2025 Reviews received at journal 23 Nov, 2025 Reviewers agreed at journal 23 Nov, 2025 Reviewers agreed at journal 22 Nov, 2025 Reviewers invited by journal 22 Nov, 2025 Editor assigned by journal 12 Nov, 2025 Submission checks completed at journal 11 Nov, 2025 First submitted to journal 11 Nov, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7905108","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":551466476,"identity":"1fa1c775-57dd-4a8a-ba07-fa096a640bac","order_by":0,"name":"Zarrindokht Emami-Karvani","email":"","orcid":"","institution":"Islamic Azad University, Isfahan","correspondingAuthor":false,"prefix":"","firstName":"Zarrindokht","middleName":"","lastName":"Emami-Karvani","suffix":""},{"id":551466478,"identity":"6e8e4171-b797-45db-a1d4-6472ede36b98","order_by":1,"name":"Giti Emtiazi","email":"","orcid":"","institution":"University of Isfahan","correspondingAuthor":false,"prefix":"","firstName":"Giti","middleName":"","lastName":"Emtiazi","suffix":""},{"id":551466485,"identity":"e3e26c3f-90a5-4da9-b574-bc700da154ad","order_by":2,"name":"Iraj Nahvi","email":"","orcid":"","institution":"University of Isfahan","correspondingAuthor":false,"prefix":"","firstName":"Iraj","middleName":"","lastName":"Nahvi","suffix":""},{"id":551466487,"identity":"3ed0513a-bf13-4236-9259-0405fd0993b9","order_by":3,"name":"Alimohammad Ahadi","email":"","orcid":"","institution":"University of Sharekord","correspondingAuthor":false,"prefix":"","firstName":"Alimohammad","middleName":"","lastName":"Ahadi","suffix":""},{"id":551466490,"identity":"02da34ae-e742-4060-ab08-f23b14831a54","order_by":4,"name":"Kianoush KHosravi-Darani","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA3klEQVRIie3RMQqDMBSA4SeBdIm4KgG9gi4upfQqkYJdukiXjoKQjs65RSdnJVAXD9CpFLyAxdWhSmmHDrHdOuSfQsgH7xEAne4PswHj5wmhEr1uyy8JZr8SID5SvXznpDi+dcPV82vS98lOgnUsDXlQEAq4DgTfBydpFlQUEuyGQdUoiAsLTs2UGSIzC2SOBC4AVTpHyMDWIiNtPxFvjoyDnSnBLMoRAToRf444GY4dwdkmRzgcd9mSoIlSJbFrHtrdwFbckm2fFEvXraW8qwh8/gUBMJRAp9PpdF/0AAtrRjj1/HStAAAAAElFTkSuQmCC","orcid":"","institution":"National Nutrition and Food Technology Research Institute","correspondingAuthor":true,"prefix":"","firstName":"Kianoush","middleName":"","lastName":"KHosravi-Darani","suffix":""}],"badges":[],"createdAt":"2025-10-20 11:23:16","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7905108/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7905108/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":97139740,"identity":"99d23770-3ff4-4fbb-aeda-894e43c4c7c0","added_by":"auto","created_at":"2025-12-01 10:02:14","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":929268,"visible":true,"origin":"","legend":"","description":"","filename":"MainCORRECTEDREF.docx","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/a919810ee0ea5a2c43031ab8.docx"},{"id":97019770,"identity":"36bfa443-bcdb-4be3-997a-29becf3d4321","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"json","order_by":1,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":6988,"visible":true,"origin":"","legend":"","description":"","filename":"cadb377bbacc4b768a126c5d796bbe6a.json","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/4f56fd23a9eeaeee3d023ab8.json"},{"id":97019776,"identity":"70062844-0ef0-4796-b2a5-4156eee1157e","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"eps","order_by":2,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":177631,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage1.eps","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/041cada422f3724a94718ff6.eps"},{"id":97140068,"identity":"a803e6ea-e1cc-4681-82ca-165ae828f535","added_by":"auto","created_at":"2025-12-01 10:03:38","extension":"eps","order_by":3,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":179199,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage2.eps","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/765f4dbd421d9b2717ce94b1.eps"},{"id":97019778,"identity":"0292fa51-b7b5-4d43-94d1-c4ad025300ca","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"eps","order_by":4,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":186511,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage3.eps","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/e8f8c816fa9f7ec94636b76d.eps"},{"id":97139543,"identity":"ee17873e-9609-4cd1-afe6-7b70cc7b103a","added_by":"auto","created_at":"2025-12-01 10:00:38","extension":"eps","order_by":5,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":180448,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage4.eps","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/c4101951a42d1a0ea2435a27.eps"},{"id":97019772,"identity":"1adf34e4-2a6c-4a96-89be-d72c0cddb26f","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"eps","order_by":6,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":117844,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage5.eps","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/ef398c5c666d9d450c61fb77.eps"},{"id":97019771,"identity":"eb5c0556-17de-45f8-87e2-55c197a8c6cf","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"png","order_by":7,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":852,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/d1fcce900b15c3b696eb86c9.png"},{"id":97138379,"identity":"b4050515-5d7a-42d8-b49b-26750860cbdf","added_by":"auto","created_at":"2025-12-01 09:58:49","extension":"png","order_by":8,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":852,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/e5e6f40ce81f5b25f5f63cce.png"},{"id":97140431,"identity":"427608b4-5971-42c4-ab31-46af4b64bf87","added_by":"auto","created_at":"2025-12-01 10:05:01","extension":"jpeg","order_by":9,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":912674,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/a18518e5e33dd0c5e2371379.jpeg"},{"id":97139155,"identity":"a996446b-4ae9-48c7-8ff4-4744b5186604","added_by":"auto","created_at":"2025-12-01 09:59:42","extension":"png","order_by":10,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":464565,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/31d4a85cf29c773af7ac71a2.png"},{"id":97138785,"identity":"a4e8d82e-6672-4170-8aac-6e9daf07c229","added_by":"auto","created_at":"2025-12-01 09:59:20","extension":"jpeg","order_by":11,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":529651,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/22586598f631d111c1af6df5.jpeg"},{"id":97019788,"identity":"40bc4c43-c715-4be9-9ce5-8b82bf6cc604","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"jpeg","order_by":12,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":398673,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage6.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/5a33ec5d98351929c0b5c373.jpeg"},{"id":97019791,"identity":"7b7f58d8-bdf1-41b3-bf87-44e4e77ed98f","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"jpeg","order_by":13,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":93994,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/f7ebb59c65cf7eaddf6c6e17.jpeg"},{"id":97019784,"identity":"356d429a-dfe7-4e6a-8f62-2bff8eb13ed7","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"png","order_by":14,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":898,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/d8c2d16f91c0fa3d0f9e1116.png"},{"id":97140749,"identity":"302102f9-79a9-40bf-ba49-f3a2c3317c0b","added_by":"auto","created_at":"2025-12-01 10:05:42","extension":"png","order_by":15,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":898,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/9a7330ec8761e055fc8bb942.png"},{"id":97019781,"identity":"ea5e7078-f499-4510-a236-7ee5c75fd26c","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"png","order_by":16,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":169171,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/fde8f48d05f864af7bd7afb2.png"},{"id":97138456,"identity":"8b776263-a964-4567-9186-7bf7f93d9aba","added_by":"auto","created_at":"2025-12-01 09:58:52","extension":"png","order_by":17,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":83517,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/b47da7e525a7efbe327f3ef0.png"},{"id":97019790,"identity":"8275edef-caad-4503-872d-ceaa22b25a01","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"png","order_by":18,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":193650,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/0719ced8fcfaa31836a32fa5.png"},{"id":97019786,"identity":"bc0db175-cd01-4348-8d93-59cc6ffcbe7f","added_by":"auto","created_at":"2025-11-28 18:02:11","extension":"png","order_by":19,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":76721,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/ff04c943242016176053c9d4.png"},{"id":97139628,"identity":"8e07ddc4-b12c-4f94-b159-80eb8609e777","added_by":"auto","created_at":"2025-12-01 10:01:24","extension":"png","order_by":20,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":60949,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1/4a6d68082105797f685467cd.png"},{"id":97145010,"identity":"0cb1a798-a028-4a2a-836b-f512042022dc","added_by":"auto","created_at":"2025-12-01 10:12:48","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":797943,"visible":true,"origin":"","legend":"","description":"","filename":"MainCORRECTEDREF.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7905108/v1_covered_b4a19f7f-f8ea-422e-af3d-361a38385814.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Microbial Desalination Using Advenella faeciporci ZF1: A Novel Iron- Reducing Bacterium Isolated from Oil Refinery Sludge","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Microbial Desalination Cells (MDCs), Iron-Reducing Bacteria (IRB), Bioelectricity generation, Electroactive Bacteria in Desalination, Microbial fuel cells (MFCs)","lastPublishedDoi":"10.21203/rs.3.rs-7905108/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7905108/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Advenella faeciporci strain ZF1, a novel isolated iron-reducing bacterium from Esfahan Oil Refining sludge, exhibited remarkable tolerance to FeCl₃ (up to 20 g/L) and electrochemical activity under aerobic and anaerobic conditions. ZF1 was phylogenetically identified and characterized via FTIR, polyhydroxybutyrate (PHB) accumulation, and magnetotactic behavior. Integrated into a microbial desalination cell (MDC), ZF1 achieved sodium, calcium, and chloride removal efficiencies of 70.6%, 78.6%, and 27.5% in refinery brine over 15 days. Under 9 g/L NaCl salinity, the system generated a maximum voltage of 410 ± 15 mV within the first 48 h. The corresponding peak power density reached 92 ± 5 mW/m² at an external resistance of 100 Ω, and the calculated current density was 1.80 ± 0.1 A/m². These findings highlight A. faeciporci ZF1 as a promising exoelectrogen for sustainable water desalination and metal-rich wastewater bioremediation.","manuscriptTitle":"Microbial Desalination Using Advenella faeciporci ZF1: A Novel Iron- Reducing Bacterium Isolated from Oil Refinery Sludge","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-28 18:02:06","doi":"10.21203/rs.3.rs-7905108/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-12-11T03:02:30+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-12-02T03:08:36+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-12-01T01:06:08+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"158769189730961688694775794509240159097","date":"2025-11-28T06:36:27+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-27T02:50:47+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"61900325469843485041211893303500611730","date":"2025-11-26T03:03:39+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"269267779069887439800228027104167827162","date":"2025-11-24T16:36:40+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-23T07:54:05+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"339019206213924065996216673009775466451","date":"2025-11-23T07:10:33+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"51101249988549126960173931013595694318","date":"2025-11-22T15:13:31+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-11-22T08:35:19+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-11-12T11:33:13+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-11-11T06:24:44+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-11-11T06:22:17+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a534f886-615f-4650-b935-59c46d9917da","owner":[],"postedDate":"November 28th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[{"id":58681901,"name":"Biological sciences/Biotechnology"},{"id":58681902,"name":"Earth and environmental sciences/Environmental sciences"},{"id":58681904,"name":"Biological sciences/Microbiology"}],"tags":[],"updatedAt":"2026-05-16T16:38:21+00:00","versionOfRecord":[],"versionCreatedAt":"2025-11-28 18:02:06","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7905108","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7905108","identity":"rs-7905108","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

Ask this paper AI returns verbatim quotes from the full text · source: preprint-html

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. This is a recent paper (2025) — citers typically take a year or two to land, and the OpenAlex reference graph may still be filling in.

Source provenance

europepmc
last seen: 2026-05-20T01:45:00.602351+00:00