Effects of Gamigwichulpajing-Tang on the Development of Experimentally-induced Endometriosis in Rats

Purpose : This study was carried out to investigate the effects of Gamigwichulpajing-Tang(GGT) on the development of experimentally-induced endometriosis in rats. Materials and Methods : Endometriosis was induced in rats by auotransplanting uterine tissue to the peritoneum and devided them into three groups: (1) sham-operated group(n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3)surgically induced endometriosis and GGT treated group. GGT(700mg/head) was orally administrated for 15days after operation. Then we measured the body weight, the volumes of endometriotic implants, the weight of uterus and ovary, and investigated the content of cytokines(MCP-1, , ) in serum and ascites. Histopathology, immunohistochemistry for COX-2, and histochemistry for mast cell in transplanted uterine tissue were performed. Results :- The of endometriotic implants in GGT-treated group was significantly decreased(p. - The content(pg/ml) of MCP-1 in ascites in GGT-treated group was significantly decreased(p. - The content(pg/ml) of in serum in GGT-treated group was significantly decreased(p. - The content(pg/ml)- of in ascites in GGT-treated group was significantly decreased(p. - The percentage(%) of positive epithelial layers for COX-2 in GGT-treated group was significantly decreased(p. - The number of mast cells in adjacent tissue of transplanted uterine tissue in GGT-treated group was significantly decreased(p. - The number of mast cells in stroma of transplanted uterine tissue in GGT-treated group was significantly decreased(p. - Histopathologically, proliferation of endomeuiotic epithelia and stroma, and infiltration of inflammatory cells in transplanted uterine tissue of GGT-treated group were weakly observed than those of control group. Conclusion :On the basis of these results, we concluded that Gamigwichulpajing-Tang have inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased Production of MCP-1 and , decreased expression of COX-2, and decreased infiltration of mast cells by administration of Gamigwichulpajing-Tang.