Analysis and occurrence of organophosphorus flame retardants and their metabolites in animal derived food

An optimized QuEChERS method for simultaneous extraction of 11 OPFRs and 7 mOPFRs in animal derived food was established and analyzed by UHPLC-MS/MS. Briefly, for beef, 4% FA/ACN was used as the extractant and Captiva EMR-Lipid was used to purify the substrate. For fish, 40% MeOH/CAN was used for extraction, followed by 200 mg PSA for purification to achieve the effective extraction of the target compounds. The method has been verified in terms of accuracy and precision. The recoveries of the method were 70%-120%, and the relative standard deviation were less than 20%. This validated method was successfully applied to determine the concentrations of the OPFRs and mOPFRs in animal derived food. The results indicated that TMP and TPrP were below the LODs in all the samples, and the remaining targets were detected to varying degrees. TCEP、TCIPP and BCEP were the primary pollutants in the fish and shrimp, indicating the transformability of OPFRs and mOPFRs. Based on the current research, exposure to OPFRs and mOPFRs through food consumption are safe to human body. However, long term exposure to OPFRs and mOPFRs still needs further study.