Anthocyanin-Rich Black Carrot (Daucus Carota Ssp. Sativus Var. Atrorubens Alef.) and Red Cabbage (Brassica Oleracea) Extracts Incorporated Biosensor for Colorimetric Detection of Helicobacter Pylori with Color Image Processing

Abstract In this work, we have developed black carrot (Daucus Carota Ssp. Sativus Var. Atrorubens Alef.) and red cabbage (Brassica Oleracea) extracts containing major anthocyanins groups incorporated a novel colorimetric biosensor for rapid, sensitive and economic detection of Helicobacter pylori (H. pylori). We comparatively used two test solutions fixed to pH 2.5 including anthocyanins rich black carrot extract (Anth@BCE) and red cabbage extract (Anth@RCE) in H. pylori and investigated their colorimetric responses based on electronic structure and electron density of anthocyanins. As an additional study, colorimetric differences observed by naked eyes were supported by color image processing with RGB (Red Green Blue) and Delta-E analysis. The findings proposed that these tests can be effectively transferred from laboratories work to clinics.