Acute Bone Damage Through Liver-bone Axis Induced by Thioacetamide in Rats

Abstract Background: Thioacetamide（TAA）is used in various fields, such as synthetic drugs, organic chemical synthesis, and materials chemistry. In the medical field, TAA is mainly used to establish animal liver injury models and other organ damage models to explore their mechanisms for helping patients with liver disease, however, TAA caused bone damage is barely understood. Therefore, the aim of our study consisted in building a rat model reflecting the TAA-treated caused acute bone damage. Results: Serum samples collected from 5-times TAA-treated rats and were used in biochemical test, we found the level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA), total bile acid (TBA), alkaline phosphatase (ALP), carbamide (UREA) and creatinine (CREA) exhibited sharply rise, while the level of serum content of total protein (TP), lactate dehydrogenase (LDH), calcium (Ca) and phosphorus (P) were severely reduced. At the same time, we obtained some data about cortical bone and trabecular bone by Micro-CT analysis, it revealed significantly decreased bone surface, tissue surface, bone volume, tissue volume in TAA-treated rats, moreover, we used a static biomechanical test system to test the femoral force range of the hind limbs of SD rats, we found bone can resist less pressure and it is easy to take fracture. Conclusions: Summarizing, our rat model presents possible mediators of liver damage, liver damage and changes in bone structure and mineralization are already visible by Micro-CT analysis after five-times of TAA treatment. The fast response and easy building possibly make it an ideal model to investigate bone metabolism in liver damage after they were affected by TAA.