Stable knockdown of attached cells with pSUPER.retro.puro vector
preprint
OA: closed
CC-BY-4.0
Abstract
Abstract This protocol describes how stable knockdown is performed using the pSUPER.retro.puro (pRS) lentiviral vector (OligoEngine) to form short hairpin RNAs for RNAi. Transient co-transfection of HEK293GP packaging cells is used to generate cell-free viral supernatants, which is then used to infect target cells. Stable shRNA production is then maintained by puromycin selection.This protocol has been optimized for attached cultured cell lines.
My notes (saved in your browser only)
Citation neighborhood (no data yet)
We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.
Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-20T11:00:21.680559+00:00
License: CC-BY-4.0