A complete chromosome substitution mapping panel reveals genome-wide epistasis in Arabidopsis

A complete chromosome substitution mapping panel reveals genome-wide epistasis in Arabidopsis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article A complete chromosome substitution mapping panel reveals genome-wide epistasis in Arabidopsis Joost Keurentjes, Cris Wijnen, Ramon Botet, Jose van de Belt, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3936850/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 09 Jul, 2024 Read the published version in Heredity → Version 1 posted 10 You are reading this latest preprint version Abstract Chromosome substitution lines (CSLs) are tentatively supreme resources to investigate non-allelic genetic interactions. However, the difficulty of generating such lines in most species largely yielded imperfect CSL panels, prohibiting a systematic dissection of epistasis. Here, we present the development and use of a unique and complete panel of CSLs in Arabidopsis thaliana, allowing the full factorial analysis of epistatic interactions. A first comparison of reciprocal single chromosome substitutions revealed a dependency of QTL detection on different genetic backgrounds. The subsequent analysis of the complete panel of CSLs enabled the mapping of the genetic interactors and identified multiple two- and three-way interactions for different traits. Some of the detected epistatic effects were as large as any observed main effect, illustrating the impact of epistasis on quantitative trait variation. We, therefore, have demonstrated the high power of detection and mapping of genome-wide epistasis, confirming the assumed supremacy of comprehensive CSL sets. Biological sciences/Genetics/Genetic interaction/Epistasis Biological sciences/Plant sciences/Plant genetics Biological sciences/Genetics/Genetic linkage study Biological sciences/Genetics/Heritable quantitative trait/Quantitative trait loci Full Text Additional Declarations There is no duality of interest Supplementary Files Supplement.pdf Supplemental Material Supplementalexceldatafiles.xlsx Data set 1 Cite Share Download PDF Status: Published Journal Publication published 09 Jul, 2024 Read the published version in Heredity → Version 1 posted Editorial decision: revise 20 May, 2024 Review # 3 received at journal 06 May, 2024 Review # 2 received at journal 26 Apr, 2024 Reviewer # 3 agreed at journal 16 Apr, 2024 Reviewer # 2 agreed at journal 06 Apr, 2024 Review # 1 received at journal 26 Mar, 2024 Reviewer # 1 agreed at journal 04 Mar, 2024 Reviewers invited by journal 04 Mar, 2024 First submitted to journal 07 Feb, 2024 Editor assigned by journal 07 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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