Human endometrium and endometriosis tissue dissociation for single-cell RNA sequencing v1

Tissue dissociation is critical step to ensure the quality of samples for single-cell RNA sequencing. To ensure recovery of most cells in the tissue without introducing stress response from the dissociation process, we adapted the dissociation method by Adam et al. (2017) and optimized for the dissociation of human endometrium and endometriosis tissue (both ectopic peritoneal and ovary lesions), targeting high quality viable cells (above 70%) with high transcript and gene counts per cell.