Potential Roles of IGF2BP3 in Bladder Cancer

The IGF2BP3 can affect cancer cells through the regulation of m6A. In this study, we combined the RNA sequencing and the RNA methylation sequencing to investigate the role and the mechanism of the IGF2BP3 in bladder cancer.TCGA data showed that the intersection genes of RBP differentially expressed and RBP involved in m6A modification were IGF2BP1 and IGF2BP3, only IGF2BP3 was significantly associated with short survival time (P < 0.01). Then immunohistochemistry and qRT-PCR detection showed that IGF2BP3 is highly expressed in bladder cancer (P < 0.05). The T24 knockout IGF2BP3 cell line was successfully constructed, with cell proliferation decreased and the apoptosis increased (P < 0.05). RNA sequencing results of clinical bladder tissue from 53 cases showed that the expression of IGF2BP3 samples were associated with the high levels of proliferation-related genes. The analysis of combined RNA and M6a level showed the differential genes related to cell growth and proliferation: SPHK1, POMT2, MRPS18a in T24 and T24-KO. Our study suggests that the IGF2BP3 is highly expressed in bladder cancer and related to tumor metastasis and invasion, the IGF2BP3 expression is related to cell proliferation genes, which affects the growth, proliferation, and apoptosis of T24 cells, and SpHK1, POMT2, and MRPS18a may be potential targets of IGF2BP3.