Comparative Analysis of the Mutational Profile in HBV-Related and Non-HBV-Related Hepatocellular Carcinoma

Abstract BackgroundHepatocellular carcinoma (HCC) is the sixth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide.The distinct molecular mechanism that regulates cancer progression associated with HBV-related HCC are still unclear.MethodsThe study population included all HCC patients from TCGA lihc_firehose.Mutational signatures of HBV-related HCC samples were calculated with MuSiCa Online website tools. Overall Survival (OS) and disease-free survival（DFS) were estimated according to the log rank in Cox proportional model.Analysis of differential expressed genes was finished with R (version 3.6.3) package DESeq2.Results AXIN1 and CACNA2D1 were significantly higher tested in patients with HBV-related HCC.Mutational signature 26,6 and 16 were detected in HBV-related HCC.TMB of non-HBV-related HCC was significantly higher than HBV-related . Mutations of TTN, MUC16, RYR2, DNAH7 and ARID2 had significant effects on OS in HBV-related but were not associated with prognosis of non-HBV-related HCC.ConclusionWnt/β-catenin and cell cycle signaling pathway may be potentially targets for treatment of HBV-related HCC.Mutation genes of TTN, MUC16, RYR2, DNAH7 and ARID2 may be used as biomarkers of the clinical prognosis and a useful strategy for management of HBV-related HCC.