DNA fingerprinting of cultivated and wild genotypes of Curcuma species from agroclimatic regions of Chhattisgarh

In the present study we had tried to investigate different genotypes of Curcuma isolated from different agroclimatic zones of Chattisgarh based on molecular fingerprinting as they contain varying range of Curcumin. Genetic fingerprints of 25 genotypes of Curcuma caesia, Curcuma longa and Curcuma aromatica by using 13 and 11 SSR primers. These primers were used to determine the diversity in genes for their proper utilization, genotypic conservation and commercial production of curcuma genotypes. Among all the studied varieties, 13 ISSR and 11SSR primers generated 107 and 51 different amplified fragments, of which 97% and 86% showed polymorphism, respectively. Through all the ISSR and SSR primers in all the genotypes amplified 1080 and 567 loci out of which 102 polymorphic and 5 monomorphic alleles were found in ISSR primers. 47 polymorphic and 4 monomorphic alleles were found in SSR. Different ISSR and SSR markers were used to estimate the DNA fingerprinting, genetic similarity and distance among or between the Curcuma genotypes. UPGMA data scoring methods were to analyze the variations between these genotypes of Curcuma . Thus, this study provides a baseline data for conservation of these curcuma genotypes that could be an important research towards optimizing industry level production of Curcumin production.