Molecular mechanism of PPMlJ regulates broiler skeletal muscle development and glycerophospholipids composition based on phosphoproteomic and metabolome.

Abstract The quality of broiler meat affects consumers’ purchasing decisions. Numerous studies have shown that phosphorylation of proteins in muscle can affect muscle quality. Here, metabolomics and transcriptomics were used to systematically identify the genetic regulation of differences in meat flavor among different broiler. By constructing the meat flavor-related metabolite-gene networks, we identified that protein phosphatase magnesium/manganese-dependent 1J (PPM1J), which is known to regulate a range of biological processes by modulating reversible protein phosphorylation, was a differentially expressed gene with the highest connectivity to meat flavor-related metabolites. PPM1J facilitated the proportion of small myofiber (≤ 250 µm2) and regulated the composition of glycerophospholipids. More importantly, phosphoproteomic and metabolome results found that PPM1J participates in the regulation of skeletal muscle development and glycerophospholipids composition by catalyzing protein dephosphorylation. Our study provides a basis for further understanding the molecular mechanism of skeletal muscle development and glycerophospholipids composition in broiler.