Model breeding and ecotoxicity study of Eprinomectin on the reproductive performance of Onthophagus hircus (Coleoptera: Scarabaeidae)

Model breeding and ecotoxicity study of Eprinomectin on the reproductive performance of Onthophagus hircus (Coleoptera: Scarabaeidae) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Model breeding and ecotoxicity study of Eprinomectin on the reproductive performance of Onthophagus hircus (Coleoptera: Scarabaeidae) Vernadet Bianchinotti, Patricia González-Vainer, Gonzalo Suárez This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5664119/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 03 Sep, 2025 Read the published version in Chemoecology → Version 1 posted 5 You are reading this latest preprint version Abstract This study developed a laboratory rearing model for the dung beetle Onthophagus hircus to evaluate the ecotoxicological effects of eprinomectin (EPM) on its survival and reproductive performance. Adult O. hircus were exposed to increasing EPM concentrations (0-50 ppm) in bovine feces. Survival was significantly reduced at 50 ppm EPM. A reproductive per-formance experiment compared control and 0.05 ppm EPM treatment groups. While adult fecundity was not significantly affected, the EPM treatment resulted in complete failure of progeny development, with no adult emergence. The study demonstrates that environmentally relevant EPM concentrations can negatively impact O. hircus populations through larvi-cidal effects, even when adult survival and reproduction appear unaffected. This highlights the importance of considering long-term impacts when assessing the environmental risks of antiparasitic drugs on beneficial dung fauna. The laboratory rearing model developed provides a valuable tool for further ecotoxicological research on dung beetles. Dung Beetles Ecotoxicology Macrocyclic lactones One Health Antiparasitic drugs Full Text Supplementary Files graphicalabstrat.sgi Cite Share Download PDF Status: Published Journal Publication published 03 Sep, 2025 Read the published version in Chemoecology → Version 1 posted Editorial decision: Major revisions 14 Mar, 2025 Reviewers agreed at journal 08 Jan, 2025 Reviewers invited by journal 18 Dec, 2024 Editor assigned by journal 18 Dec, 2024 First submitted to journal 17 Dec, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5664119","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":391868601,"identity":"334fb338-3c9b-4302-bbec-87c9ed76e832","order_by":0,"name":"Vernadet Bianchinotti","email":"","orcid":"","institution":"Universidad de la Republica Uruguay Facultad de Veterinaria","correspondingAuthor":false,"prefix":"","firstName":"Vernadet","middleName":"","lastName":"Bianchinotti","suffix":""},{"id":391868602,"identity":"b6bb5a21-0ca2-4524-a9f7-3abb4f4a5f3a","order_by":1,"name":"Patricia González-Vainer","email":"","orcid":"","institution":"Universidad de la Republica Uruguay Facultad de Ciencias","correspondingAuthor":false,"prefix":"","firstName":"Patricia","middleName":"","lastName":"González-Vainer","suffix":""},{"id":391868603,"identity":"2299f263-4284-49cc-a292-5f99d3715b52","order_by":2,"name":"Gonzalo Suárez","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA8klEQVRIiWNgGAWjYDADNjBZAcTMzA2kaDkD0sJIpBYwYGwDk/i1yM9Ifvbhw5/DcnzSh589+DivNpq/HajlR8U2nFoMbqQZz5zBc9iYjS/N3HDmtuO5Mw4zNjD2nLmNW4tEgjEzj0RaYhsPg5k077ZjuQ1ALcyMbbi1yM9I/8z8xyCtvo2H/Zs075xjufMJaWG4kWPMzJBgk8DGwwO0paEmdwMhLQZn3hQz9hywMWzj4SmTnHHsQO5GoJaD+Pwi356+meHHHwl5+R72bRIfaupy550/fPDBjwo8DkMDh8HkAaLVA0EdKYpHwSgYBaNghAAAmepTxqbrFJwAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0003-2452-3546","institution":"Universidad de la Republica Uruguay Facultad de Veterinaria","correspondingAuthor":true,"prefix":"","firstName":"Gonzalo","middleName":"","lastName":"Suárez","suffix":""}],"badges":[],"createdAt":"2024-12-17 18:25:26","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5664119/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5664119/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s00049-025-00426-9","type":"published","date":"2025-09-03T15:56:55+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":90828013,"identity":"9e8c2365-c64f-40c9-8313-c5f6fd087515","added_by":"auto","created_at":"2025-09-08 16:05:00","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":463475,"visible":true,"origin":"","legend":"","description":"","filename":"submissionmanuscriptBianchinotti.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5664119/v1_covered_d1289027-f89d-4b9c-a932-b45220f18846.pdf"},{"id":71970637,"identity":"d6b9043d-b875-40a2-9fe9-f9a0f22cfdf8","added_by":"auto","created_at":"2024-12-20 08:19:15","extension":"sgi","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":495622,"visible":true,"origin":"","legend":"","description":"","filename":"graphicalabstrat.sgi","url":"https://assets-eu.researchsquare.com/files/rs-5664119/v1/dd45c8d2713240ef18e0e4e1.sgi"}],"financialInterests":"","formattedTitle":"Model breeding and ecotoxicity study of Eprinomectin on the reproductive performance of Onthophagus hircus (Coleoptera: Scarabaeidae)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"chemoecology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"chem","sideBox":"Learn more about [Chemoecology](https://www.springer.com/journal/49)","snPcode":"49","submissionUrl":"https://submission.nature.com/new-submission/49/3","title":"Chemoecology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Dung Beetles, Ecotoxicology, Macrocyclic lactones, One Health, Antiparasitic drugs","lastPublishedDoi":"10.21203/rs.3.rs-5664119/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5664119/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"This study developed a laboratory rearing model for the dung beetle Onthophagus hircus to evaluate the ecotoxicological effects of eprinomectin (EPM) on its survival and reproductive performance. 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