Polysaccharide of Atractylodes Macrocephala Koidz(PAMK) Enhance the Immunomodulatory Activity of RAW264.7 Through TLR4/MyD88/NF-κB Pathway

The Polysaccharide of Atractylodes macrocephala Koidz (PAMK) has been reported to play an important role in improving the immune function of mammals and poultry. But the molecular mechanism in cell is still not unclear. For investigating the mechanism in mice, RAW264.7 were cultured and divided into six groups (cell, NC, siTLR4, NC + PAMK, PAMK, siTLR4 + PAMK). The serum cytokines, mRNA relative expression level of cytokines and TLR4 signal pathway, the protein expression level of TLR4 signal pathway were measured. The results showed PAMK could increase the level of IL-4, IL-6, TNF-α, IFN-γ, and increase the mRNA relative expression level and protein expression level of TLR4, MyD88, IKK, NF-κB. In conclusion, PAMK may regulate the immue activity of RAW264.7 through the TLR4/MyD88/NF-κB pathway.