Comparative Genomics and Phylogenetic Analysis of the Complete Mitochondrial Genome of Siphonariidae

Comparative Genomics and Phylogenetic Analysis of the Complete Mitochondrial Genome of Siphonariidae | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 26 November 2025 V1 Latest version Share on Comparative Genomics and Phylogenetic Analysis of the Complete Mitochondrial Genome of Siphonariidae Authors : zhiming xiang 0000-0003-1428-2910 [email protected] , liu lu 0009-0001-3116-1187 , li ru , zhao congxin , lin ruixin , xiao shu , mao fan , Yang Zhang , Yuehuan Zhang , and Ziniu Yu Authors Info & Affiliations https://doi.org/10.22541/au.176414668.80755946/v1 287 views 141 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Siphonariidae is an important family within Tectipleura (Heterobranchia) and is currently the subject of ongoing debate about its morphological classification. Although mitochondria are powerful tools for phylogenetic research, the limited availability of published mitochondrial genome data for species within this family has constrained the improvement of their phylogenetic relationships. To clarify the mitochondrial genome characteristics and phylogenetic status of Siphonariidae, this study included Illumina high-throughput sequencing for the sequencing and analysis of the complete mitochondrial genome of Siphonaria sp. scsio1106 from the Xisha Islands, Hainan, China, and the results were compared with previously published Tectipleura mitogenomes. The mitochondrial genome of Siphonaria sp. scsio1106 is 13,809 base pairs in length and contains 37 genes, indicating an AT bias of 67.06%. The coding region constitutes 97.47% of the genome, indicating a compact structure. The Ka/Ks ratios for the 13 protein-coding genes (PCGs) were less than 1. Phylogenetic analysis based on concatenated 16S rRNA sequences of Tectipleura revealed a close relationship with Siphonaria sp. scsio1106. Divergence time estimation indicated that Siphonariidae diverged from other Tectipleura species approximately 158.6 million years ago (Mya), suggesting that Siphonariidae represents a more recently diverged lineage within Tectipleura. Analysis of gene arrangements revealed three events in the mitochondrial genomes of S. gigas and Siphonaria sp. scsio1106. Our research provides new insights into the evolution of Siphonariidae through phylogenetic analysis, offering valuable data for understanding the evolution and functional organization of mitochondrial genomes within Tectipleura. Supplementary Material File (manuscription.docx) Download 129.99 KB File (table1-organization of the genome.docx) Download 27.61 KB File (table2-taxonomic details and genbank accession numbers of the mitogenomes used in phylogenetic analysis.xlsx) Download 14.64 KB Information & Authors Information Version history V1 Version 1 26 November 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords experimental evolution invertebrate marine molecular evolution Authors Affiliations zhiming xiang 0000-0003-1428-2910 [email protected] South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author liu lu 0009-0001-3116-1187 South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author li ru South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author zhao congxin South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author lin ruixin South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author xiao shu South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author mao fan South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author Yang Zhang South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author Yuehuan Zhang South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author Ziniu Yu South China Sea Institute of Oceanology Chinese Academy of Sciences View all articles by this author Metrics & Citations Metrics Article Usage 287 views 141 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation zhiming xiang, liu lu, li ru, et al. Comparative Genomics and Phylogenetic Analysis of the Complete Mitochondrial Genome of Siphonariidae. Authorea . 26 November 2025. DOI: https://doi.org/10.22541/au.176414668.80755946/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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