A Zea mays alanyl-tRNA synthetase mutant is seedling-lethal and suggests a retrograde signal from plastid rrn16 leader derived lncRNAs

A Zea mays alanyl-tRNA synthetase mutant is seedling-lethal and suggests a retrograde signal from plastid rrn16 leader derived lncRNAs | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article A Zea mays alanyl-tRNA synthetase mutant is seedling-lethal and suggests a retrograde signal from plastid rrn16 leader derived lncRNAs Anuradha Dhingra, Indra Adhikari, Chris Rock This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5699627/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract [120 words] A predicted retrograde signal for photomorphogenesis appears conserved with humans: organellar-homologous rRNA fragments mapping to mis-regulated long non-coding nuclear and/or mitochondrial pseudogene transcripts. Systems analysis of a maize virescent mutant for a plastid-localized ala-tRNA synthetase suggests histone genes as a novel retrograde signaling target in addition to other photobleaching-affected processes like Fe chelator transporters, terpene/chlorophyll and tetrapyrrole biosynthesis, glutathionylation, amino acid catabolism for energy, vesicle trafficking, and TORC/abiotic energy stress effectors. We speculate that deranged polyadenylation as an extreme photobleaching response associated with degradation of the retrograde signal might account for an observed bias in the most highly expressed nuclear genes: up typically in chlorotic photosynthetic mutants versus abnormally extreme bias for down-regulation of those genes in null albino mutants. Main Text [ 2170 words ] corn bulk segregant resequencing tRNA synthetase histones TOR-C signaling Full Text Additional Declarations The authors declare no competing interests. Table 1 is available in the supplementary files section. Supplementary Files albinomsSupplTables021025PNASready.xlsx Supplemental Tables S1- S6 PNAStemplateSIdhingra031225finalrev.docx Supplemental Text and Figures S1- S12 Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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