Selenite Serves as a Versatile Proxy for Phosphate Homeostasis Studies

Selenite Serves as a Versatile Proxy for Phosphate Homeostasis Studies | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Selenite Serves as a Versatile Proxy for Phosphate Homeostasis Studies Wenyuan Ruan, Qianqian Zhang, Xueqing Wang, Dagang Tian, Tian Zhou, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6806744/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Phosphate (Pi) homeostasis is essential for energy metabolism, signaling, and structural integrity across eukaryotes and prokaryotes, yet current methods for studying Pi dynamics remain limited. Here, we demonstrate that selenite serves as a versatile tool to address three major challenges: assessing Pi status, quantifying Pi uptake capacity, and enabling high-throughput screening of Pi regulators. Selenite-induced growth responses exhibit superior specificity for Pi availability compared to other nutrients, permitting rapid evaluation of Pi uptake efficiency and visual phenotyping of Pi-deficient organisms through growth differentials. This approach circumvents the need for conventional platforms while bypassing safety and regulatory constraints. As proof of concept, we identified HPA6/SPS11—a previously uncharacterized Pi regulator that functionally integrates sucrose biosynthesis, Pi homeostasis, and Pi-associated immune responses in rice. By combining quantitative accuracy with phenotypic simplicity, selenite will emerge as a transformative reagent for elucidating Pi regulatory networks across biological systems. Biological sciences/Biotechnology/Plant biotechnology Biological sciences/Plant sciences/Plant physiology Phosphate (Pi) Selenite Pi uptake Pi homeostasis Method Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupplementaryTableS1.Primersusedinthisstudy.xlsx Primers used in this study SupplementaryTableS2.GOtermslistoftheDEGsinhpa6mutant.xlsx GO terms list of the DEGs in the hpa6 mutant Supplementaryfile1.CodefornetworkanlaysisofthePrelatedpapperspubllishedsince1950s.txt Code for network analysis of the P-related papers published since 1950s Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6806744","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":469983930,"identity":"9fea2d01-faaf-4c98-bfb3-a685c053e75f","order_by":0,"name":"Wenyuan 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