Exploring the effects of different processing techniques on the antitussive expectorant of Platycodon grandiflorus by metabo-nomics and pharmacologic

BACKGROUND AND PURPOSE: Platycodon grandiflorum is a traditional Chinese herbal medi-cine, which can disperse lung, promote pharynx, dispel phlegm and expel pus. Its processing history was first seen in the “Zhou Hou Bei Ji Fang“ of the Jin Dynasty in China. Through pro-cessing, effective substances in plants can be enriched and negative substances can be reduced. Therefore, this study explored the effects of different processing methods on the antitussive and expectorant effects of Platycodon grandiflorum through metabolomics and pharmacology, aiming to provide a theoretical basis for clinical application. EXPERIMENTAL APPROACH: The changes of metabolites before and after processing of Platycodon grandiflorum were analyzed by Nexera UHPLC LC-30A-HILIC-Orbitrap-Q Exactive HF-X platform. The antitussive and expectorant effects of processed Platycodon grandiflorum were verified by mouse concentrated ammonia induced cough method and tracheal phenol red drainage method. KEY RESULTS: After processing, some metabolites of Platycodon grandiflorum changed, among which 24 kinds of terpenoid metabolites changed significantly. Through the experiment of am-monia-induced cough in mice and phenol red expectorant experiment, it was found that fresh JZI 40 %, fresh CZ 80 %, dry JZZ 40 % and dry JZI 80 % had obvious antitussive effect, and dry JZZ 40 % had obvious expectorant effect. CONCLUSIONS AND IMPLICATIONS: Some processing methods can indeed further improve the antitussive and expectorant effects of Platycodon grandiflorum, which may be due to the ef-fect of increasing the content of triterpenoids after processing, but the mechanism of processing to improve the effect of relieving cough and expectorant needs further study.