Physiological responses and proteomic analysis of different barley varieties under alkaline stress

Physiological responses and proteomic analysis of different barley varieties under alkaline stress | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Physiological responses and proteomic analysis of different barley varieties under alkaline stress Zhe Li, Jianbo Li, Yanan Jin, Tu Ba, Ersuo Lv, Zhiping Liu, Shoujun Xu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7393472/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 02 Apr, 2026 Read the published version in Scientific Reports → Version 1 posted 16 You are reading this latest preprint version Abstract Soil salinization threatens agricultural development, and understanding the alkali tolerance mechanism of barley is crucial for breeding. Using the alkali-tolerant barley variety Jia-HY460-6R and the alkali-sensitive variety Noire Maroc as materials, morphological and physiological indicators were determined and proteomic analysis was conducted after treatment with 100 mmol·L⁻¹ Na₂CO₃:NaHCO₃. The results showed that Jia-HY460-6R was superior to Noire Maroc in growth indicators, antioxidant capacity, ion balance, and osmotic regulation. A total of 1359 differentially expressed proteins were identified, and 27 key proteins were screened, involving multiple metabolic pathways such as the MAPK signaling pathway. This study provides a basis for understanding the molecular mechanism of barley alkali tolerance and breeding. Biological sciences/Biochemistry Biological sciences/Plant sciences Barley proteomics༛ alkaline stress༛ metabolic pathway Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 02 Apr, 2026 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 20 Oct, 2025 Reviews received at journal 17 Oct, 2025 Reviews received at journal 14 Oct, 2025 Reviews received at journal 11 Oct, 2025 Reviews received at journal 30 Sep, 2025 Reviewers agreed at journal 29 Sep, 2025 Reviews received at journal 24 Sep, 2025 Reviewers agreed at journal 24 Sep, 2025 Reviewers agreed at journal 24 Sep, 2025 Reviewers agreed at journal 24 Sep, 2025 Reviewers agreed at journal 24 Sep, 2025 Reviewers invited by journal 11 Sep, 2025 Editor assigned by journal 09 Sep, 2025 Editor invited by journal 09 Sep, 2025 Submission checks completed at journal 04 Sep, 2025 First submitted to journal 04 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7393472","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":514762826,"identity":"62453b50-d08a-4031-9dec-7337fd55f2e2","order_by":0,"name":"Zhe Li","email":"","orcid":"","institution":"College of Agriculture, Inner Mongolia Minzu University","correspondingAuthor":false,"prefix":"","firstName":"Zhe","middleName":"","lastName":"Li","suffix":""},{"id":514762829,"identity":"eac1b242-23ec-4a08-a6de-07ed3c20d2f9","order_by":1,"name":"Jianbo Li","email":"","orcid":"","institution":"College of Agriculture, Inner Mongolia Minzu 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Using the alkali-tolerant barley variety Jia-HY460-6R and the alkali-sensitive variety Noire Maroc as materials, morphological and physiological indicators were determined and proteomic analysis was conducted after treatment with 100 mmol\u0026middot;L⁻\u0026sup1; Na₂CO₃:NaHCO₃. The results showed that Jia-HY460-6R was superior to Noire Maroc in growth indicators, antioxidant capacity, ion balance, and osmotic regulation. A total of 1359 differentially expressed proteins were identified, and 27 key proteins were screened, involving multiple metabolic pathways such as the MAPK signaling pathway. 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