Plasma Metagenomic Cell-Free DNA Sequencing Identifies Clinically Unsuspected Cases of Toxoplasma gondii Infection in Immunocompromised Patients

2025 · doi:10.1099/acmi.0.001068.v1
preprint OA: closed
Full text JSON View at publisher

Abstract

Toxoplasmosis is challenging to diagnose. Here we describe eight cases of T. gondii infections identified in immunocompromised hosts by plasma metagenomic cell-free deoxyribonucleic acid (cfDNA) sequencing, including five clinically unsuspected cases, and review the potential for plasma cfDNA sequencing to expand the diagnosis of toxoplasmosis in diverse clinical presentations.
Full text 910 characters · extracted from oa-doi-fallback · click to expand
Full text loading... Abstract Toxoplasmosis is challenging to diagnose. Here we describe eight cases of T. gondii infections identified in immunocompromised hosts by plasma metagenomic cell-free deoxyribonucleic acid (cfDNA) sequencing, including five clinically unsuspected cases, and review the potential for plasma cfDNA sequencing to expand the diagnosis of toxoplasmosis in diverse clinical presentations. - Received: - Version Posted: Funding - University of Minnesota Foundation - Principal Award Recipient: Lea R. Goren - Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases (Award 5T32AI05543318) - Principal Award Recipient: Alice C. Lehman - Centers for Disease Control and Prevention - Principal Award Recipient: Alice C. Lehman - National Center for Advancing Translational Sciences (Award UL1TR002494) - Principal Award Recipient: Not Applicable

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. This is a recent paper (2025) — citers typically take a year or two to land, and the OpenAlex reference graph may still be filling in.

Source provenance

europepmc
last seen: 2026-05-20T01:45:00.602351+00:00