Edibological Study on the Mealworm, Tenebrio molitor with Utilizing the White Albino Mice as a Biomarker Reference

Edibological Study on the Mealworm, Tenebrio molitor with Utilizing the White Albino Mice as a Biomarker Reference | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Edibological Study on the Mealworm, Tenebrio molitor with Utilizing the White Albino Mice as a Biomarker Reference Khaled S. M. Osman, Mohammed Z. Y. Aly, Ibrahim I. I. Mohamed, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8290871/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Edible insects are recognized as a valuable nutritional source and a promising alternative to traditional feed ingredients. The primary objective of this study was to compare and optimize the rearing conditions of Tenebrio molitor on two diets and to use white albino mice as biomarker references for the T. molitor diet. For that purpose, T. molitor was reared on dried sunflower seeds (diet A) and wheat bran (control diet), using two treatments, frozen (FM) and dry matter (DM), before running biochemical analyses. Then, exploiting the T. molitor diet for mice and comparing them with traditional diets for five weeks, using weight measurement, total proteins, lipids, and carbohydrates as biomarker references. T. molitor / diet A showed significantly higher carbohydrate and lipid levels (110.3 ± 1.45 and 51.5 ± 1.97 mg/g FM), respectively. Otherwise, the highest weight of mice groups was recorded in the T. molitor diet group (44.34 ± 2.27 g). Likewise, the T. molitor diet group recorded high content of protein and carbohydrate (8.26 and 3.24 g/100ml), respectively. The results suggest that insects can be an effective and sustainable replacement for traditional feed in mice diets. They support healthy and sustained growth, making them a promising option for animal nutrition and sustainable agriculture. Biological sciences/Biochemistry Biological sciences/Biotechnology Biological sciences/Physiology Biological sciences/Plant sciences Biological sciences/Zoology Edible insect Tenebrio molitor White albino mice sunflower seeds wheat bran Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8290871","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":581022184,"identity":"118d84f7-c48f-425e-89c8-ea738d0da4d4","order_by":0,"name":"Khaled S. M. Osman","email":"","orcid":"","institution":"Qena University","correspondingAuthor":false,"prefix":"","firstName":"Khaled","middleName":"S. M.","lastName":"Osman","suffix":""},{"id":581022185,"identity":"9b296b2f-6f6b-4377-8c2d-38764cf89b12","order_by":1,"name":"Mohammed Z. Y. 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The primary objective of this study was to compare and optimize the rearing conditions of \u003cem\u003eTenebrio molitor\u003c/em\u003e on two diets and to use white albino mice as biomarker references for the \u003cem\u003eT. molitor\u003c/em\u003e diet. For that purpose, \u003cem\u003eT. molitor\u003c/em\u003e was reared on dried sunflower seeds (diet A) and wheat bran (control diet), using two treatments, frozen (FM) and dry matter (DM), before running biochemical analyses. Then, exploiting the \u003cem\u003eT. molitor\u003c/em\u003e diet for mice and comparing them with traditional diets for five weeks, using weight measurement, total proteins, lipids, and carbohydrates as biomarker references. \u003cem\u003eT. molitor\u003c/em\u003e/ diet A showed significantly higher carbohydrate and lipid levels (110.3\u0026thinsp;\u0026plusmn;\u0026thinsp;1.45 and 51.5\u0026thinsp;\u0026plusmn;\u0026thinsp;1.97 mg/g FM), respectively. Otherwise, the highest weight of mice groups was recorded in the \u003cem\u003eT. molitor\u003c/em\u003e diet group (44.34\u0026thinsp;\u0026plusmn;\u0026thinsp;2.27 g). Likewise, the \u003cem\u003eT. molitor\u003c/em\u003e diet group recorded high content of protein and carbohydrate (8.26 and 3.24 g/100ml), respectively. The results suggest that insects can be an effective and sustainable replacement for traditional feed in mice diets. They support healthy and sustained growth, making them a promising option for animal nutrition and sustainable agriculture.\u003c/p\u003e","manuscriptTitle":"Edibological Study on the Mealworm, Tenebrio molitor with Utilizing the White Albino Mice as a Biomarker Reference","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-01-28 15:30:37","doi":"10.21203/rs.3.rs-8290871/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"009a4cc0-a046-4201-8442-930e47c533f6","owner":[],"postedDate":"January 28th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":61806063,"name":"Biological sciences/Biochemistry"},{"id":61806064,"name":"Biological sciences/Biotechnology"},{"id":61806065,"name":"Biological sciences/Physiology"},{"id":61806066,"name":"Biological sciences/Plant sciences"},{"id":61806067,"name":"Biological sciences/Zoology"}],"tags":[],"updatedAt":"2026-04-08T20:54:09+00:00","versionOfRecord":[],"versionCreatedAt":"2026-01-28 15:30:37","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8290871","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8290871","identity":"rs-8290871","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}