Study of danazol active agenteffect on Mcf10a breast cells redoxphosphorylation

Relevance: Fibrocystic mastopathy is one of the most complex breast pathologies, occurring in about 60% of women. The causes and molecular mechanisms of cyst formation in fibrocystic mastopathy are not fully understood, and effective treatment methods are not widely implemented. However, there is evidence of the fibrocystic mastopathy malignization risk. We studied the molecular effect of the active agent danazol on the respiratory complex in mitochondria, which occur in various concentrations and are considered the cell energy center. A method of permeation with special substances was developed to improve the cell membrane permeability in the intracellular proteins and nucleic acids analysis. The scientists aimed to study the Mcf10a cell line mitochondrial respiratory complex using the permeabilization method. Purpose: To study the effect of different danazol concentrations on Mcf10a breast cells redox phosphorylation in women with fibrocystic mastopathy. Results: Monitoring the redox phosphorylation process using the respiratory complex substrates affecting the Msf10A breast cells respiratory chain showed the danazol 30 μM effect on the respiratory complex I. Conclusions: According to oxygraphic records, 10 minutes’ exposure to danazol 30 μM after permeabilization with digitonin increased the Msf10A cells’ oxidative phosphorylation compared with the control. The respiratory rate decreased by 45% ten minutes after treatment with digitonin.