Optimization of fermentation parameters for the production of antimicrobial compounds by Fusarium falciforme MNP1 isolated from rhizosphere soil of Epipremnum aureum

Optimization of fermentation parameters for the production of antimicrobial compounds by Fusarium falciforme MNP1 isolated from rhizosphere soil of Epipremnum aureum | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Short Report Optimization of fermentation parameters for the production of antimicrobial compounds by Fusarium falciforme MNP1 isolated from rhizosphere soil of Epipremnum aureum Rani Nisha, Jain Pranay This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9243931/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In the present study, Fusarium falciforme MNP1 (Gen Bank Accession no. PX106850) was isolated from the rhizosphere soil of Epipremnum aureum (Money Plant), which showed the remarkable antimicrobial activity against the test microorganisms ( Escherichia coli MTCC- 40, Pseudomonas aeruginosa MTCC-647, Bacillus subtilis MTCC- 2274, Staphylococcus aureus MTCC-6908, Aspergillus niger MTCC- 281 and Candida albicans MTCC- 183). One Factor at a time (OFAT) and Response Surface Methodology (RSM) were used for the optimization of the fermentation parameters for antimicrobial production. In the OFAT design, fermentation parameters such as pH (4–8), temperature (15–35℃), incubation period (7–15 days), inoculum size (0.25–1.25 ml) and fermentation vessel size (250–2000 ml) were considered. Response surface methodology (RSM) based central composite design (CCD) model was performed to evaluate the interactions and the quadratic effect on the antimicrobial metabolites production which inhibit the test microorganisms (responses) in terms of the diameter of zone of growth inhibition (mm). Three variables namely incubation time (days), pH, and temperature (℃) were employed with RSM and investigated with the help of quadratic equations to increase the production of bioactive metabolites. The experimental data to maximize the production of bioactive metabolite was significantly fit by using the second polynomial model. Regression analysis revealed that the model was found significant on the basis R 2 values for all the six responses and values of adjusted R 2 demonstrated the perfect agreement with predicted and experimental values. Methodology (RSM) enhanced the production of bioactive compounds by Fusarium falciforme with optimized conditions pH 6, 25℃ temperature and 9 days of incubation period. By using the central composite design, the accurate values of fermentation parameters for the production of antimicrobial compound can be concluded and further characterization of the antimicrobial metabolites is needed for future prospect. Rhizosphere fungi OFAT RSM Optimization Fermentation Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9243931","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Short Report","associatedPublications":[],"authors":[{"id":613783498,"identity":"4c7b644c-cb88-428c-ba69-894d801817e3","order_by":0,"name":"Rani Nisha","email":"data:image/png;base64,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","orcid":"","institution":"University Institute of Engineering and Technology, Kurukshetra University","correspondingAuthor":true,"prefix":"","firstName":"Rani","middleName":"","lastName":"Nisha","suffix":""},{"id":613783501,"identity":"4cb43b2f-68bf-4f7e-944c-1bb9f2126625","order_by":1,"name":"Jain Pranay","email":"","orcid":"","institution":"University Institute of Engineering and Technology, Kurukshetra University","correspondingAuthor":false,"prefix":"","firstName":"Jain","middleName":"","lastName":"Pranay","suffix":""}],"badges":[],"createdAt":"2026-03-27 11:08:18","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9243931/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9243931/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":105904701,"identity":"e6e1bc8b-ca99-4864-ba09-b8d9f6421f66","added_by":"auto","created_at":"2026-04-01 10:10:19","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1293694,"visible":true,"origin":"","legend":"","description":"","filename":"RSMpaper1.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9243931/v1_covered_42c73a8c-cb20-4045-b026-16366dddada7.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Optimization of fermentation parameters for the production of antimicrobial compounds by Fusarium falciforme MNP1 isolated from rhizosphere soil of Epipremnum aureum","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Rhizosphere, fungi, OFAT, RSM, Optimization, Fermentation","lastPublishedDoi":"10.21203/rs.3.rs-9243931/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9243931/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn the present study, \u003cem\u003eFusarium falciforme\u003c/em\u003e MNP1 (Gen Bank Accession no. PX106850) was isolated from the rhizosphere soil of \u003cem\u003eEpipremnum aureum\u003c/em\u003e (Money Plant), which showed the remarkable antimicrobial activity against the test microorganisms (\u003cem\u003eEscherichia coli\u003c/em\u003e MTCC- 40, \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e MTCC-647, \u003cem\u003eBacillus subtilis\u003c/em\u003e MTCC- 2274, \u003cem\u003eStaphylococcus aureus\u003c/em\u003e MTCC-6908, \u003cem\u003eAspergillus niger\u003c/em\u003e MTCC- 281 and \u003cem\u003eCandida albicans\u003c/em\u003e MTCC- 183). One Factor at a time (OFAT) and Response Surface Methodology (RSM) were used for the optimization of the fermentation parameters for antimicrobial production. In the OFAT design, fermentation parameters such as pH (4\u0026ndash;8), temperature (15\u0026ndash;35℃), incubation period (7\u0026ndash;15 days), inoculum size (0.25\u0026ndash;1.25 ml) and fermentation vessel size (250\u0026ndash;2000 ml) were considered. Response surface methodology (RSM) based central composite design (CCD) model was performed to evaluate the interactions and the quadratic effect on the antimicrobial metabolites production which inhibit the test microorganisms (responses) in terms of the diameter of zone of growth inhibition (mm). Three variables namely incubation time (days), pH, and temperature (℃) were employed with RSM and investigated with the help of quadratic equations to increase the production of bioactive metabolites. The experimental data to maximize the production of bioactive metabolite was significantly fit by using the second polynomial model. Regression analysis revealed that the model was found significant on the basis R\u003csup\u003e2\u003c/sup\u003e values for all the six responses and values of adjusted R\u003csup\u003e2\u003c/sup\u003e demonstrated the perfect agreement with predicted and experimental values. Methodology (RSM) enhanced the production of bioactive compounds by \u003cem\u003eFusarium falciforme\u003c/em\u003e with optimized conditions pH 6, 25℃ temperature and 9 days of incubation period. By using the central composite design, the accurate values of fermentation parameters for the production of antimicrobial compound can be concluded and further characterization of the antimicrobial metabolites is needed for future prospect.\u003c/p\u003e","manuscriptTitle":"Optimization of fermentation parameters for the production of antimicrobial compounds by Fusarium falciforme MNP1 isolated from rhizosphere soil of Epipremnum aureum","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-03-31 18:39:32","doi":"10.21203/rs.3.rs-9243931/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"dd2ac988-6461-4b82-a75f-724be9a9be06","owner":[],"postedDate":"March 31st, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-03-31T18:40:18+00:00","versionOfRecord":[],"versionCreatedAt":"2026-03-31 18:39:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-9243931","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-9243931","identity":"rs-9243931","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}